| Host: | Rabbit |
| Applications: | WB/ELISA/IHC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Target of rapamycin complex 2 subunit MAPKAP1 (193-243 aa) is suitable for use in Western Blot, ELISA and Immunohistochemistry research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC-P 1:50-300 ELISA 2000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPKAP1 |
| Gene ID: | 79109 |
| Uniprot ID: | SIN1_HUMAN |
| Immunogen Region: | 193-243 aa |
| Specificity: | This antibody detects endogenous levels of SIN1 at Human/Mouse/Rat |
| Immunogen: | Synthesized peptide derived from the human SIN1 at the amino acid range 193-243 |
| Function | Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex. |
| Protein Name | Target Of Rapamycin Complex 2 Subunit Mapkap1 Torc2 Subunit Mapkap1 Mitogen-Activated Protein Kinase 2-Associated Protein 1 Stress-Activated Map Kinase-Interacting Protein 1 Sapk-Interacting Protein 1 Msin1 |
| Database Links | Reactome: R-HSA-1257604 Reactome: R-HSA-389357 Reactome: R-HSA-5218920 Reactome: R-HSA-5674400 Reactome: R-HSA-6804757 |
| Cellular Localisation | Cell Membrane Peripheral Membrane Protein Cytoplasmic Vesicle Nucleus |
| Alternative Antibody Names | Anti-Target Of Rapamycin Complex 2 Subunit Mapkap1 antibody Anti-Torc2 Subunit Mapkap1 antibody Anti-Mitogen-Activated Protein Kinase 2-Associated Protein 1 antibody Anti-Stress-Activated Map Kinase-Interacting Protein 1 antibody Anti-Sapk-Interacting Protein 1 antibody Anti-Msin1 antibody Anti-MAPKAP1 antibody Anti-MIP1 antibody Anti-SIN1 antibody |
Information sourced from Uniprot.org
