| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-JNK2 (1-424) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAPK9 |
| Gene ID: |
5601 |
| Uniprot ID: |
MK09_HUMAN |
| Immunogen Region: |
1-424 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-424 of human JNK2 (NP_002743.3). |
| Immunogen Sequence: |
MSDSKCDSQFYSVQVADSTF TVLKRYQQLKPIGSGAQGIV CAAFDTVLGINVAVKKLSRP FQNQTHAKRAYRELVLLKCV NHKNIISLLNVFTPQKTLEE FQDVYLVMELMDANLCQVIH MELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVK SDCTLKILDFGLARTACTNF MMTPYVVTRYYRAPEVILGM GYKENVDIWSVGCIMGELVK GCVIFQGTDHIDQWNKVIE |
| Post Translational Modifications | Dually phosphorylated on Thr-183 and Tyr-185 by MAP2K7 and MAP2K4, which activates the enzyme. Autophosphorylated in vitro. |
| Function | Serine/threonine-protein kinase involved in various processes such as cell proliferation, differentiation, migration, transformation and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK9/JNK2. In turn, MAPK9/JNK2 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. In response to oxidative or ribotoxic stresses, inhibits rRNA synthesis by phosphorylating and inactivating the RNA polymerase 1-specific transcription initiation factor RRN3. Promotes stressed cell apoptosis by phosphorylating key regulatory factors including TP53 and YAP1. In T-cells, MAPK8 and MAPK9 are required for polarized differentiation of T-helper cells into Th1 cells. Upon T-cell receptor (TCR) stimulation, is activated by CARMA1, BCL10, MAP2K7 and MAP3K7/TAK1 to regulate JUN protein levels. Plays an important role in the osmotic stress-induced epithelial tight-junctions disruption. When activated, promotes beta-catenin/CTNNB1 degradation and inhibits the canonical Wnt signaling pathway. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the regulation of the circadian clock. Phosphorylates POU5F1, which results in the inhibition of POU5F1's transcriptional activity and enhances its proteasomal degradation. MAPK9 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to JUN, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it. |
| Protein Name | Mitogen-Activated Protein Kinase 9Map Kinase 9Mapk 9Jnk-55Stress-Activated Protein Kinase 1aSapk1aStress-Activated Protein Kinase Jnk2C-Jun N-Terminal Kinase 2 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2871796Reactome: R-HSA-450321Reactome: R-HSA-450341 |
| Cellular Localisation | CytoplasmNucleusColocalizes With Pou5f1 In The Nucleus |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 9 antibodyAnti-Map Kinase 9 antibodyAnti-Mapk 9 antibodyAnti-Jnk-55 antibodyAnti-Stress-Activated Protein Kinase 1a antibodyAnti-Sapk1a antibodyAnti-Stress-Activated Protein Kinase Jnk2 antibodyAnti-C-Jun N-Terminal Kinase 2 antibodyAnti-MAPK9 antibodyAnti-JNK2 antibodyAnti-PRKM9 antibodyAnti-SAPK1A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
