• Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (STJ11101473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse brain cells using 3 Mu g JNK1/2/3 antibody (STJ11101473). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (STJ11101473) at a dilution of 1:1000.

Anti-MAPK8/MAPK9/MAPK10 antibody (1-370) (STJ11101473)

SKU:
STJ11101473

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/IP/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cells
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK10
MAPK9
5602
5601
5599
MK10_HUMAN
MK09_HUMAN
MK08_HUMAN
Immunogen Region: 1-370
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1).
Immunogen Sequence: MSRSKRDNNFYSVEIGDSTF TVLKRYQNLKPIGSGAQGIV CAAYDAILERNVAIKKLSRP FQNQTHAKRAYRELVLMKCV NHKNIIGLLNVFTPQKSLEE FQDVYIVMELMDANLCQVIQ MELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVK SDCTLKILDFGLARTAGTSF MMTPYVVTRYYRAPEVILGM GYKENVDLWSVGCIMGEMVC HKILFPGRDYIDQWNKVIE
Tissue Specificity
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody
Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance