• Western blot analysis of extracts of various cell lines, using JNK1/2/3 antibody (STJ11101473) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded human colon using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse spleen using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U2OS cells using JNK1/2/3 rabbit polyclonal antibody (STJ11101473) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 600 Mu g extracts of mouse brain cells using 3 Mu g JNK1/2/3 antibody (STJ11101473). Western blot was performed from the immunoprecipitate using JNK1/2/3 antibody (STJ11101473) at a dilution of 1:1000.

Anti-MAPK8/MAPK9/MAPK10 antibody (1-370) (STJ11101473)

SKU:
STJ11101473

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-JNK1/2/3 (1-370) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK9
MAPK8
5601
5599
5602
MK09_HUMAN
MK08_HUMAN
MK10_HUMAN
Immunogen Region: 1-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human JNK1/2/3 (NP_620637.1).
Immunogen Sequence: MSRSKRDNNFYSVEIGDSTF TVLKRYQNLKPIGSGAQGIV CAAYDAILERNVAIKKLSRP FQNQTHAKRAYRELVLMKCV NHKNIIGLLNVFTPQKSLEE FQDVYIVMELMDANLCQVIQ MELDHERMSYLLYQMLCGIK HLHSAGIIHRDLKPSNIVVK SDCTLKILDFGLARTAGTSF MMTPYVVTRYYRAPEVILGM GYKENVDLWSVGCIMGEMVC HKILFPGRDYIDQWNKVIE
Tissue Specificity P45984 - NA
P45983 - NA
P53779 - Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
P45984 - NA
P45983 - NA
P53779 - Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney.
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody
Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody
Anti-C-Jun N-Terminal Kinase 1 antibody
Anti-MAPK8 antibody
Anti-JNK1 antibody
Anti-PRKM8 antibody
Anti-SAPK1 antibody
Anti-SAPK1C antibody
Anti-Mitogen-Activated Protein Kinase 10 antibody
Anti-Map Kinase 10 antibody
Anti-Mapk 10 antibody
Anti-Map Kinase P49 3f12 antibody
Anti-Stress-Activated Protein Kinase 1b antibody
Anti-Sapk1b antibody
Anti-Stress-Activated Protein Kinase Jnk3 antibody
Anti-C-Jun N-Terminal Kinase 3 antibody
Anti-MAPK10 antibody
Anti-JNK3 antibody
Anti-JNK3A antibody
Anti-PRKM10 antibody
Anti-SAPK1B antibody
Anti-Mitogen-Activated Protein Kinase 9 antibody
Anti-Map Kinase 9 antibody
Anti-Mapk 9 antibody
Anti-Jnk-55 antibody
Anti-Stress-Activated Protein Kinase 1a antibody
Anti-Sapk1a antibody
Anti-Stress-Activated Protein Kinase Jnk2 antibody
Anti-C-Jun N-Terminal Kinase 2 antibody
Anti-MAPK9 antibody
Anti-JNK2 antibody
Anti-PRKM9 antibody
Anti-SAPK1A antibody
Anti-Mitogen-Activated Protein Kinase 8 antibody
Anti-Map Kinase 8 antibody
Anti-Mapk 8 antibody
Anti-Jnk-46 antibody
Anti-Stress-Activated Protein Kinase 1c antibody
Anti-Sapk1c antibody
Anti-Stress-Activated Protein Kinase Jnk1 antibody

Information sourced from Uniprot.org

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