| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MAPK7 (1-815) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAPK7 |
| Gene ID: |
5598 |
| Uniprot ID: |
MK07_HUMAN |
| Immunogen Region: |
1-815 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-815 of human ERK5 (NP_002740.2). |
| Immunogen Sequence: |
MAEPLKEEDGEDGSAEPPGP VKAEPAHTAASVAAKNLALL KARSFDVTFDVGDEYEIIET IGNGAYGVVSSARRRLTGQQ VAIKKIPNAFDVVTNAKRTL RELKILKHFKHDNIIAIKDI LRPTVPYGEFKSVYVVLDLM ESDLHQIIHSSQPLTLEHVR YFLYQLLRGLKYMHSAQVIH RDLKPSNLLVNENCELKIGD FGMARGLCTSPAEHQYFMTE YVATRWYRAPELMLSLHEY |
| Tissue Specificity | Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver. |
| Post Translational Modifications | Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent. |
| Function | Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction. |
| Protein Name | Mitogen-Activated Protein Kinase 7Map Kinase 7Mapk 7Big Map Kinase 1Bmk-1Extracellular Signal-Regulated Kinase 5Erk-5 |
| Database Links | Reactome: R-HSA-198753Reactome: R-HSA-198765Reactome: R-HSA-202670Reactome: R-HSA-2559582Reactome: R-HSA-881907Reactome: R-HSA-8853659 |
| Cellular Localisation | CytoplasmNucleusPml BodyTranslocates To The Nucleus Upon Activation |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 7 antibodyAnti-Map Kinase 7 antibodyAnti-Mapk 7 antibodyAnti-Big Map Kinase 1 antibodyAnti-Bmk-1 antibodyAnti-Extracellular Signal-Regulated Kinase 5 antibodyAnti-Erk-5 antibodyAnti-MAPK7 antibodyAnti-BMK1 antibodyAnti-ERK5 antibodyAnti-PRKM7 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
