| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: | Rabbit polyclonal antibody anti-Mitogen-activated protein kinase 6 (461-510 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPK6 |
| Gene ID: | 5597 |
| Uniprot ID: | MK06_HUMAN |
| Immunogen Region: | 461-510 aa |
| Specificity: | ERK 3 Polyclonal Antibody detects endogenous levels of ERK 3 protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human p97 MAPK at the amino acid range 461-510 |
| Post Translational Modifications | Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway. |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle. |
| Protein Name | Mitogen-Activated Protein Kinase 6 Map Kinase 6 Mapk 6 Extracellular Signal-Regulated Kinase 3 Erk-3 Map Kinase Isoform P97 P97-Mapk |
| Database Links | Reactome: R-HSA-5687128 |
| Cellular Localisation | Cytoplasm Nucleus Translocates To The Cytoplasm Following Interaction With Mapkapk5 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 6 antibody Anti-Map Kinase 6 antibody Anti-Mapk 6 antibody Anti-Extracellular Signal-Regulated Kinase 3 antibody Anti-Erk-3 antibody Anti-Map Kinase Isoform P97 antibody Anti-P97-Mapk antibody Anti-MAPK6 antibody Anti-ERK3 antibody Anti-PRKM6 antibody |
Information sourced from Uniprot.org
