• Western blot analysis of the lysates from HeLa cells using p97 MAPK antibody.
  • Western blot analysis of lysates from K562 cells, using p97 MAPK Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Immunofluorescence analysis of MCF7 cells, using p97 MAPK Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of Hela Lysate, antibody was diluted at 1:1000. Secondary antibody was diluted at 1:20000

Anti-MAPK6 antibody (461-510 aa) (STJ92993)

SKU:
STJ92993

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Mitogen-activated protein kinase 6 (461-510 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAPK6
Gene ID: 5597
Uniprot ID: MK06_HUMAN
Immunogen Region: 461-510 aa
Specificity: ERK 3 Polyclonal Antibody detects endogenous levels of ERK 3 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human p97 MAPK at the amino acid range 461-510
Post Translational Modifications Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
Function Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle.
Protein Name Mitogen-Activated Protein Kinase 6
Map Kinase 6
Mapk 6
Extracellular Signal-Regulated Kinase 3
Erk-3
Map Kinase Isoform P97
P97-Mapk
Database Links Reactome: R-HSA-5687128
Cellular Localisation Cytoplasm
Nucleus
Translocates To The Cytoplasm Following Interaction With Mapkapk5
Alternative Antibody Names Anti-Mitogen-Activated Protein Kinase 6 antibody
Anti-Map Kinase 6 antibody
Anti-Mapk 6 antibody
Anti-Extracellular Signal-Regulated Kinase 3 antibody
Anti-Erk-3 antibody
Anti-Map Kinase Isoform P97 antibody
Anti-P97-Mapk antibody
Anti-MAPK6 antibody
Anti-ERK3 antibody
Anti-PRKM6 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance