| Host: | Rabbit |
| Applications: | WB/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S2MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MAPK10 |
| Gene ID: | 5602 |
| Uniprot ID: | MK10_HUMAN |
| Immunogen Region: | 350-450 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 350-450 of human MAPK10 (P53779). |
| Immunogen Sequence: | VDDALQHPYINVWYDPAEVE APPPQIYDKQLDEREHTIEE WKELIYKEVMNSEEKTKNGV VKGQPSPSGAAVNSSESLPP SSSVNDISSMSTDQTLASDT D |
| Tissue Specificity | Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney. |
| Post Translational Modifications | Dually phosphorylated on Thr-221 and Tyr-223 by MAP2K4 and MAP2K7, which activates the enzyme. MAP2K7 shows a strong preference for Thr-221 while MAP2K4 phosphorylates Tyr-223 preferentially. Weakly autophosphorylated on threonine and tyrosine residues in vitro. Palmitoylation regulates subcellular location and axonal development. |
| Function | Serine/threonine-protein kinase involved in various processes such as neuronal proliferation, differentiation, migration and programmed cell death. Extracellular stimuli such as pro-inflammatory cytokines or physical stress stimulate the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. In this cascade, two dual specificity kinases MAP2K4/MKK4 and MAP2K7/MKK7 phosphorylate and activate MAPK10/JNK3. In turn, MAPK10/JNK3 phosphorylates a number of transcription factors, primarily components of AP-1 such as JUN and ATF2 and thus regulates AP-1 transcriptional activity. Plays regulatory roles in the signaling pathways during neuronal apoptosis. Phosphorylates the neuronal microtubule regulator STMN2. Acts in the regulation of the amyloid-beta precursor protein/APP signaling during neuronal differentiation by phosphorylating APP. Participates also in neurite growth in spiral ganglion neurons. Phosphorylates the CLOCK-BMAL1 heterodimer and plays a role in the photic regulation of the circadian clock. Phosphorylates JUND and this phosphorylation is inhibited in the presence of MEN1. |
| Protein Name | Mitogen-Activated Protein Kinase 10 Map Kinase 10 Mapk 10 Map Kinase P49 3f12 Stress-Activated Protein Kinase 1b Sapk1b Stress-Activated Protein Kinase Jnk3 C-Jun N-Terminal Kinase 3 |
| Database Links | Reactome: R-HSA-2559580 Reactome: R-HSA-2871796 Reactome: R-HSA-450321 Reactome: R-HSA-450341 |
| Cellular Localisation | Cytoplasm Membrane Lipid-Anchor Nucleus Mitochondrion Palmitoylation Regulates Mapk10 Trafficking To Cytoskeleton Recruited To The Mitochondria In The Presence Of Sarm1 |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase 10 antibody Anti-Map Kinase 10 antibody Anti-Mapk 10 antibody Anti-Map Kinase P49 3f12 antibody Anti-Stress-Activated Protein Kinase 1b antibody Anti-Sapk1b antibody Anti-Stress-Activated Protein Kinase Jnk3 antibody Anti-C-Jun N-Terminal Kinase 3 antibody Anti-MAPK10 antibody Anti-JNK3 antibody Anti-JNK3A antibody Anti-PRKM10 antibody Anti-SAPK1B antibody |
Information sourced from Uniprot.org