Anti-MAP4K2 antibody (400-480 aa) (STJ191699)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 400-480
General Information
| Short Description | Rabbit polyclonal anti-Mitogen-activated protein kinase kinase kinase kinase 2 (400-480 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MAP4K2 |
| Gene ID | 5871 |
| Uniprot ID | M4K2_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 400-480 |
| Immunogen Region | 400-480 aa |
| Specificity | M4K2 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Polyubiquitinated through 'Lys-48'-polyubiquitin chains, allowing proteasomal turnover. Ubiquitination requires the kinase activity of MAP4K2/GCK. Autophosphorylated in response to tumor necrosis factor (TNF), endotoxins or pro-inflammatory stimuli. Autophosphorylation leads to activation. |
| Function | Serine/threonine-protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Acts as a MAPK kinase kinase kinase (MAP4K) and is an upstream activator of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway and to a lesser extent of the p38 MAPKs signaling pathway. Required for the efficient activation of JNKs by TRAF6-dependent stimuli, including pathogen-associated molecular patterns (PAMPs) such as polyinosine-polycytidine (poly(IC)), lipopolysaccharides (LPS), lipid A, peptidoglycan (PGN), or bacterial flagellin. To a lesser degree, IL-1 and engagement of CD40 also stimulate MAP4K2-mediated JNKs activation. The requirement for MAP4K2/GCK is most pronounced for LPS signaling, and extends to LPS stimulation of c-Jun phosphorylation and induction of IL-8. Enhances MAP3K1 oligomerization, which may relieve N-terminal mediated MAP3K1 autoinhibition and lead to activation following autophosphorylation. Also mediates the SAP/JNK signaling pathway and the p38 MAPKs signaling pathway through activation of the MAP3Ks MAP3K10/MLK2 and MAP3K11/MLK3. May play a role in the regulation of vesicle targeting or fusion. regulation of vesicle targeting or fusion. Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway. |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2B Lymphocyte Serine/Threonine-Protein KinaseGerminal Center KinaseGc KinaseMapk/Erk Kinase Kinase Kinase 2Mek Kinase Kinase 2Mekkk 2Rab8-Interacting Protein |
| Database Links | |
| Cellular Localisation | CytoplasmBasolateral Cell MembranePeripheral Membrane ProteinGolgi Apparatus Membrane |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase Kinase 2 antibodyAnti-B Lymphocyte Serine/Threonine-Protein Kinase antibodyAnti-Germinal Center Kinase antibodyAnti-Gc Kinase antibodyAnti-Mapk/Erk Kinase Kinase Kinase 2 antibodyAnti-Mek Kinase Kinase 2 antibodyAnti-Mekkk 2 antibodyAnti-Rab8-Interacting Protein antibodyAnti-MAP4K2 antibodyAnti-GCK antibodyAnti-RAB8IP antibody |
Information sourced from Uniprot.org