| Host: |
Rabbit |
| Applications: |
IHC |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ASK1 (1173-1374) is suitable for use in Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAP3K5 |
| Gene ID: |
4217 |
| Uniprot ID: |
M3K5_HUMAN |
| Immunogen Region: |
1173-1374 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1173-1374 of human ASK1 (NP_005914.1). |
| Immunogen Sequence: |
ELRPHFSLASESDTADQEDL DVEDDHEEQPSNQTVRRPQA VIEDAVATSGVSTLSSTVSH DSQSAHRSLNVQLGRMKIET NRLLEELVRKEKELQALLHR AIEEKDQEIKHLKLKSQPIE IPELPVFHLNSSGTNTEDSE LTDWLRVNGADEDTISRFLA EDYTLLDVLYYVTRDDLKCL RLRGGMLCTLWKAIIDFRNK QT |
| Tissue Specificity | Abundantly expressed in heart and pancreas. |
| Post Translational Modifications | Phosphorylated at Thr-838 through autophosphorylation and by MAP3K6/ASK2 which leads to activation. Thr-838 is dephosphorylated by PPP5C. Ser-83 and Ser-1033 are inactivating phosphorylation sites, the former of which is phosphorylated by AKT1. Phosphorylated at Ser-966 which induces association of MAP3K5/ASK1 with the 14-3-3 family proteins and suppresses MAP3K5/ASK1 activity. Calcineurin (CN) dephosphorylates this site. Also dephosphorylated and activated by PGAM5. Phosphorylation at Ser-966 in response to oxidative stress is negatively regulated by PPIA/CYPA. Ubiquitinated. Tumor necrosis factor (TNF) induces TNFR2-dependent ubiquitination, leading to proteasomal degradation. Ubiquitinated by RC3H2 in a TRIM48-dependent manner. Methylation at Arg-78 and Arg-80 by PRMT1 promotes association of MAP3K5 with thioredoxin and negatively regulates MAP3K5 association with TRAF2, inhibiting MAP3K5 activation. Methylation is blocked by ubiquitination of PRMT1 by TRIM48. |
| Function | Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. Plays an important role in the cascades of cellular responses evoked by changes in the environment. Mediates signaling for determination of cell fate such as differentiation and survival. Plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation. MAP3K5/ASK1 is required for the innate immune response, which is essential for host defense against a wide range of pathogens. Mediates signal transduction of various stressors like oxidative stress as well as by receptor-mediated inflammatory signals, such as the tumor necrosis factor (TNF) or lipopolysaccharide (LPS). Once activated, acts as an upstream activator of the MKK/JNK signal transduction cascade and the p38 MAPK signal transduction cascade through the phosphorylation and activation of several MAP kinase kinases like MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6 and MAP2K7/MKK7. These MAP2Ks in turn activate p38 MAPKs and c-jun N-terminal kinases (JNKs). Both p38 MAPK and JNKs control the transcription factors activator protein-1 (AP-1). |
| Protein Name | Mitogen-Activated Protein Kinase Kinase Kinase 5Apoptosis Signal-Regulating Kinase 1Ask-1Mapk/Erk Kinase Kinase 5Mek Kinase 5Mekk 5 |
| Database Links | Reactome: R-HSA-2559580 |
| Cellular Localisation | CytoplasmEndoplasmic ReticulumInteraction With 14-3-3 Proteins Alters The Distribution Of Map3k5/Ask1 And Restricts It To The Perinuclear Endoplasmic Reticulum Region |
| Alternative Antibody Names | Anti-Mitogen-Activated Protein Kinase Kinase Kinase 5 antibodyAnti-Apoptosis Signal-Regulating Kinase 1 antibodyAnti-Ask-1 antibodyAnti-Mapk/Erk Kinase Kinase 5 antibodyAnti-Mek Kinase 5 antibodyAnti-Mekk 5 antibodyAnti-MAP3K5 antibodyAnti-ASK1 antibodyAnti-MAPKKK5 antibodyAnti-MEKK5 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
