• Western blot analysis of extracts of mouse brain, using MAP2 rabbit polyclonal antibody antibody (STJ114887) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using MAP2 antibody (STJ114887) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using MAP2 antibody (STJ114887) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of mouse brain cells using MAP2 rabbit polyclonal antibody (STJ114887) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using MAP2 rabbit polyclonal antibody (STJ114887) at dilution of 1:20 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAP2 antibody (1-118) (STJ114887)

SKU:
STJ114887

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Map2 (1-118) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAP2
Gene ID: 4133
Uniprot ID: MTAP2_HUMAN
Immunogen Region: 1-118
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-118 of human MAP2 (NP_002365.3).
Immunogen Sequence: MADERKDEAKAPHWTSAPLT EASAHSHPPEIKDQGGAGEG LVRSANGFPYREDEEGAFGE HGSQGTYSNTKENGINGELT SADRETAEEVSARIVQVVTA EAVAVLKGEQEKEAQHKD
Post Translational Modifications Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly. Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation.
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Protein Name Microtubule-Associated Protein 2
Map-2
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Dendrite
Alternative Antibody Names Anti-Microtubule-Associated Protein 2 antibody
Anti-Map-2 antibody
Anti-MAP2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance