| Host: |
Sheep |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Sheep polyclonal antibody anti-MAP1LC3A is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised |
| Purification: |
Affinity purification |
| Dilution Range: |
IHC, WB. Use at a dilution of 1:200 to 1:2000. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
MAP1LC3A |
| Gene ID: |
84557 |
| Uniprot ID: |
MLP3A_HUMAN |
| Specificity: |
Specific for MAP1LC3A. |
| Immunogen: |
A synthetic peptide from human microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A, MAP1A/MAP1B LC3 A) conjugated to blue carrier protein has been used as the antigen. |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). |
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. |
| Protein Name | Microtubule-Associated Proteins 1a/1b Light Chain 3aAutophagy-Related Protein Lc3 AAutophagy-Related Ubiquitin-Like Modifier Lc3 AMap1 Light Chain 3-Like Protein 1Map1a/Map1b Light Chain 3 AMap1a/Map1b Lc3 AMicrotubule-Associated Protein 1 Light Chain 3 Alpha |
| Database Links | Reactome: R-HSA-1632852Reactome: R-HSA-5205685Reactome: R-HSA-8934903 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic Membranes |
| Alternative Antibody Names | Anti-Microtubule-Associated Proteins 1a/1b Light Chain 3a antibodyAnti-Autophagy-Related Protein Lc3 A antibodyAnti-Autophagy-Related Ubiquitin-Like Modifier Lc3 A antibodyAnti-Map1 Light Chain 3-Like Protein 1 antibodyAnti-Map1a/Map1b Light Chain 3 A antibodyAnti-Map1a/Map1b Lc3 A antibodyAnti-Microtubule-Associated Protein 1 Light Chain 3 Alpha antibodyAnti-MAP1LC3A antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
