• Immunohistochemistry analysis of MANBA in paraffin-embedded human colon carcinoma using MANBA Rabbit polyclonal antibody (STJ117484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MANBA in paraffin-embedded human thyroid cancer using MANBA Rabbit polyclonal antibody (STJ117484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MANBA in paraffin-embedded human liver cancer using MANBA Rabbit polyclonal antibody (STJ117484) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MANBA Rabbit polyclonal antibody (STJ117484) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MANBA antibody (18-300) (STJ117484)

SKU:
STJ117484

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MANBA (18-300) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MANBA
Gene ID: 4126
Uniprot ID: MANBA_HUMAN
Immunogen Region: 18-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 18-300 of human MANBA (NP_005899.3).
Immunogen Sequence: AAELSYSLRGNWSICNGNGS LELPGAVPGCVHSALFQQGL IQDSYYRFNDLNYRWVSLDN WTYSKEFKIPFEISKWQKVN LILEGVDTVSKILFNEVTIG ETDNMFNRYSFDITNVVRDV NSIELRFQSAVLYAAQQSKA HTRYQVPPDCPPLVQKGECH VNFVRKEQCSFSWDWGPSFP TQGIWKDVRIEAYNICHLNY FTFSPIYDKSAQEWNLEIES TFDVVSSKPVGGQVIVAIP
Tissue Specificity Ubiquitous. Detected in pancreas, kidney and placenta, ands at lower levels in liver, lung, brain, heart and muscle.
Function Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.
Protein Name Beta-Mannosidase
Lysosomal Beta A Mannosidase
Mannanase
Mannase
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-8853383
Cellular Localisation Lysosome
Alternative Antibody Names Anti-Beta-Mannosidase antibody
Anti-Lysosomal Beta A Mannosidase antibody
Anti-Mannanase antibody
Anti-Mannase antibody
Anti-MANBA antibody
Anti-MANB1 antibody

Information sourced from Uniprot.org

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