Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4ยฐC overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-MAF1 antibody (110-190 aa) (STJ93986)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from Maf1 at the amino acid range 110-190
STJ93986
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General Information

Short DescriptionRabbit polyclonal anti-Repressor of RNA polymerase III transcription MAF1 homolog (110-190 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsIHC/IF/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeIHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
FormulationLiquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMAF1
Gene ID84232
Uniprot IDMAF1_HUMAN
ImmunogenSynthesized peptide derived from Maf1 at the amino acid range 110-190
Immunogen Region110-190 aa
SpecificityMaf1 Polyclonal Antibody detects endogenous levels of Maf1 protein.

Additional Info

Function Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance. Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation. Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP.
Protein Name Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog
Database Links Reactome: R-HSA-8943724
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog antibody
Anti-MAF1 antibody

Information sourced from Uniprot.org

Citations

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