| Host: |
Rabbit |
| Applications: |
IHC/IF/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Repressor of RNA polymerase III transcription MAF1 homolog (110-190 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MAF1 |
| Gene ID: |
84232 |
| Uniprot ID: |
MAF1_HUMAN |
| Immunogen Region: |
110-190 aa |
| Specificity: |
Maf1 Polyclonal Antibody detects endogenous levels of Maf1 protein. |
| Immunogen: |
Synthesized peptide derived from Maf1 at the amino acid range 110-190 |
| Function | Plays a role in the repression of RNA polymerase III-mediated transcription in response to changing nutritional, environmental and cellular stress conditions to balance the production of highly abundant tRNAs, 5S rRNA, and other small non-coding RNAs with cell growth and maintenance. Also plays a key role in cell fate determination by promoting mesorderm induction and adipocyte differentiation. Mechanistically, associates with the RNA polymerase III clamp and thereby impairs its recruitment to the complex made of the promoter DNA, TBP and the initiation factor TFIIIB. When nutrients are available and mTOR kinase is active, MAF1 is hyperphosphorylated and RNA polymerase III is engaged in transcription. Stress-induced MAF1 dephosphorylation results in nuclear localization, increased targeting of gene-bound RNA polymerase III and a decrease in the transcriptional readout. Additionally, may also regulate RNA polymerase I and RNA polymerase II-dependent transcription through its ability to regulate expression of the central initiation factor TBP. |
| Protein Name | Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog |
| Database Links | Reactome: R-HSA-8943724 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Repressor Of Rna Polymerase Iii Transcription Maf1 Homolog antibodyAnti-MAF1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
