• Immunofluorescence analysis of U2OS cells using MAD1/MAD1/MAD1L1 antibody (STJ24440).
  • Western blot analysis of extracts of various cell lines, using MAD1/MAD1/MAD1L1 antibody (STJ24440) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.

Anti-MAD1L1 antibody (360-700) (STJ24440)

SKU:
STJ24440

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Host: Rabbit
Applications: WB/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MAD1L1 (360-700) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MAD1L1
Gene ID: 8379
Uniprot ID: MD1L1_HUMAN
Immunogen Region: 360-700
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1/MAD1/MAD1L1 (NP_001013859.1).
Immunogen Sequence: RGLEKARQQLQEELRQVSGQ LLEERKKRETHEALARRLQK RVLLLTKERDGMRAILGSYD SELTPAEYSPQLTRRMREAE DMVQKVHSHSAEMEAQLSQA LEELGGQKQRADMLEMELKM LKSQSSSAEQSFLFSREEAD TLRLKVEELEGERSRLEEEK RMLEAQLERRALQGDYDQSR TKVLHMSLNPTSVARQRLRE DHSQLQAECERLRGLLRAME RGGTVPADLEAAAASLPSS
Tissue Specificity Isoform 1: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level). Isoform 3: Expressed in hepatocellular carcinomas and hepatoma cell lines (at protein level).
Post Translational Modifications Phosphorylated.by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. Phosphorylated.by TTK.
Function Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Forms a heterotetrameric complex with the closed conformation form of MAD2L1 (C-MAD2) at unattached kinetochores during prometaphase, recruits an open conformation of MAD2L1 (O-MAD2) and promotes the conversion of O-MAD2 to C-MAD2, which ensures mitotic checkpoint signaling. Isoform 3: Sequesters MAD2L1 in the cytoplasm preventing its function as an activator of the mitotic spindle assembly checkpoint (SAC) resulting in SAC impairment and chromosomal instability in hepatocellular carcinomas.
Protein Name Mitotic Spindle Assembly Checkpoint Protein Mad1
Mitotic Arrest Deficient 1-Like Protein 1
Mad1-Like Protein 1
Mitotic Checkpoint Mad1 Protein Homolog
Hsmad1
Hmad1
Tax-Binding Protein 181
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Cellular Localisation Nucleus
Chromosome
Centromere
Kinetochore
Nucleus Envelope
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
Co-Localizes With Tpr At The Nucleus Envelope During Interphase And Throughout The Cell Cycle
From The Beginning To The End Of Mitosis
It Is Seen To Move From A Diffusely Nuclear Distribution To The Centrosome
To The Spindle Midzone And Finally To The Midbody
Localizes To Kinetochores During Prometaphase
Does Not Localize To Kinetochores During Metaphase
Colocalizes With Nek2 At The Kinetochore
Colocalizes With Ik At Spindle Poles During Metaphase And Anaphase
Isoform 3: Cytoplasm
Alternative Antibody Names Anti-Mitotic Spindle Assembly Checkpoint Protein Mad1 antibody
Anti-Mitotic Arrest Deficient 1-Like Protein 1 antibody
Anti-Mad1-Like Protein 1 antibody
Anti-Mitotic Checkpoint Mad1 Protein Homolog antibody
Anti-Hsmad1 antibody
Anti-Hmad1 antibody
Anti-Tax-Binding Protein 181 antibody
Anti-MAD1L1 antibody
Anti-MAD1 antibody
Anti-TXBP181 antibody

Information sourced from Uniprot.org

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