• Western blot analysis of extracts of various cell lines, using Lysozyme (Lysozyme (LYZ) ) antibody (STJ115472) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Lysozyme (LYZ) antibody (STJ115472) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using Lysozyme (LYZ) rabbit polyclonal antibody (STJ115472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse intestin using Lysozyme (LYZ) rabbit polyclonal antibody (STJ115472) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of RAW264. 7 cells using Lysozyme (LYZ) rabbit monoclonal antibody (STJ115472) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of THP-1 cells using Lysozyme (LYZ) rabbit monoclonal antibody (STJ115472) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-LYZ antibody (1-100) (STJ115472)

SKU:
STJ115472

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LYZ (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYZ
Gene ID: 4069
Uniprot ID: LYSC_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Lysozyme (Lysozyme (LYZ) ) (NP_000230.1).
Immunogen Sequence: MKALIVLGLVLLSVTVQGKV FERCELARTLKRLGMDGYRG ISLANWMCLAKWESGYNTRA TNYNAGDRSTDYGIFQINSR YWCNDGKTPGAVNACHLSCS
Function Lysozymes have primarily a bacteriolytic function.those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
Protein Name Lysozyme C
1 -4-Beta-N-Acetylmuramidase C
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6803157
Reactome: R-HSA-977225
Cellular Localisation Secreted
Alternative Antibody Names Anti-Lysozyme C antibody
Anti-1 -4-Beta-N-Acetylmuramidase C antibody
Anti-LYZ antibody
Anti-LZM antibody

Information sourced from Uniprot.org

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