Anti-LYAR antibody (241-290 aa) (STJ93971)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human LYAR at the amino acid range 241-290
General Information
| Short Description | Rabbit polyclonal anti-Cell growth-regulating nucleolar protein (241-290 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LYAR |
| Gene ID | 55646 |
| Uniprot ID | LYAR_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human LYAR at the amino acid range 241-290 |
| Immunogen Region | 241-290 aa |
| Specificity | LYAR Polyclonal Antibody detects endogenous levels of LYAR protein. |
Additional Info
| Function | Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal. |
| Protein Name | Cell Growth-Regulating Nucleolar Protein |
| Database Links | |
| Cellular Localisation | NucleusNucleolusCytoplasmCell ProjectionCiliumPhotoreceptor Outer SegmentComponent Of Pre-Ribosomal ParticlesIncluding Pre-40sPre-60s And Pre-90sAssociated With Cytoplasmic RibosomesBut Not PolysomesAs A Component Of The 60s SubunitIn The RetinaPredominantly Expressed In Photoreceptor Outer SegmentsIn The NucleolusColocalizes With Nucleolin/NclTherefore May Reside In The Dense Fibrillar Component (Dfc) |
| Alternative Antibody Names | Anti-Cell Growth-Regulating Nucleolar Protein antibodyAnti-LYAR antibodyAnti-PNAS-5 antibody |
Information sourced from Uniprot.org