• Western blot analysis of the lysates from K562 cells using LYAR antibody.
  • Western blot analysis of SH-SY5Y cells using LYAR Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of lysates from Jurkat, HepG2, and LOVO cells, using LYAR Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using LYAR Polyclonal Antibody diluted at 1：2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-LYAR antibody (241-290 aa) (STJ93971)

SKU:
STJ93971

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Cell growth-regulating nucleolar protein (241-290 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LYAR
Gene ID: 55646
Uniprot ID: LYAR_HUMAN
Immunogen Region: 241-290 aa
Specificity: LYAR Polyclonal Antibody detects endogenous levels of LYAR protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human LYAR at the amino acid range 241-290
Function Plays a role in the maintenance of the appropriate processing of 47S/45S pre-rRNA to 32S/30S pre-rRNAs and their subsequent processing to produce 18S and 28S rRNAs. Also acts at the level of transcription regulation. Along with PRMT5, binds the gamma-globin (HBG1/HBG2) promoter and represses its expression. In neuroblastoma cells, may also repress the expression of oxidative stress genes, including CHAC1, HMOX1, SLC7A11, ULBP1 and SNORD41 that encodes a small nucleolar RNA. Preferentially binds to a DNA motif containing 5'-GGTTAT-3'. Negatively regulates the antiviral innate immune response by targeting IRF3 and impairing its DNA-binding activity. In addition, inhibits NF-kappa-B-mediated expression of pro-inflammatory cytokines. Stimulates phagocytosis of photoreceptor outer segments by retinal pigment epithelial cells. Prevents nucleolin/NCL self-cleavage, maintaining a normal steady-state level of NCL protein in undifferentiated embryonic stem cells (ESCs), which in turn is essential for ESC self-renewal.
Protein Name Cell Growth-Regulating Nucleolar Protein
Cellular Localisation Nucleus
Nucleolus
Cytoplasm
Cell Projection
Cilium
Photoreceptor Outer Segment
Component Of Pre-Ribosomal Particles
Including Pre-40s
Pre-60s And Pre-90s
Associated With Cytoplasmic Ribosomes
But Not Polysomes
As A Component Of The 60s Subunit
In The Retina
Predominantly Expressed In Photoreceptor Outer Segments
In The Nucleolus
Colocalizes With Nucleolin/Ncl
Therefore May Reside In The Dense Fibrillar Component (Dfc)
Alternative Antibody Names Anti-Cell Growth-Regulating Nucleolar Protein antibody
Anti-LYAR antibody
Anti-PNAS-5 antibody

Information sourced from Uniprot.org

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