• Immunohistochemistry analysis of paraffin-embedded mouse kidney using Lumican (LUM) (LUM) Rabbit monoclonal antibody (STJ11101364) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using Lumican (LUM) (LUM) Rabbit monoclonal antibody (STJ11101364) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Lumican (LUM) (LUM) Rabbit monoclonal antibody (STJ11101364) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LUM antibody (212-338) [S4MR] (STJ11101364)

SKU:
STJ11101364

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Lumican (212-338) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LUM
Gene ID: 4060
Uniprot ID: LUM_HUMAN
Immunogen Region: 212-338
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 212-338 of human Lumican (LUM) (NP_002336.1).
Immunogen Sequence: LDNNKISNIPDEYFKRFNAL QYLRLSHNELADSGIPGNSF NVSSLVELDLSYNKLKNIPT VNENLENYYLEVNQLEKFDI KSFCKILGPLSYSKIKHLRL DGNRISETSLPPDMYECLRV ANEVTLN
Tissue Specificity Cornea and other tissues.
Post Translational Modifications Sulfated on tyrosine residue(s). Contains keratan sulfate.
Protein Name Lumican
Keratan Sulfate Proteoglycan Lumican
Kspg Lumican
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-2022857
Reactome: R-HSA-216083
Reactome: R-HSA-3000178
Reactome: R-HSA-3656225
Reactome: R-HSA-3656243
Reactome: R-HSA-3656244
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Lumican antibody
Anti-Keratan Sulfate Proteoglycan Lumican antibody
Anti-Kspg Lumican antibody
Anti-LUM antibody
Anti-LDC antibody
Anti-SLRR2D antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance