• Immunofluorescence analysis of U-2 OS cells using LRRFIP2 Rabbit polyclonal antibody (STJ11102837) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using LRRFIP2 Rabbit polyclonal antibody (STJ11102837) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-LRRFIP2 antibody (150-340) (STJ11102837)

SKU:
STJ11102837

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRRFIP2
Gene ID: 9209
Uniprot ID: LRRF2_HUMAN
Immunogen Region: 150-340
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 150-340 of human LRRFIP2 (NP_060194.1).
Immunogen Sequence: VIEEQEEQMAEFYRENEEKS KELERQKHMCSVLQHKMEEL KEGLRQRDELIEKHGLVIIP DGTPNGDVSHEPVAGAITVV SQEAAQVLESAGEGPLDVRL RKLAGEKEELLSQIRKLKLQ LEEERQKCSRNDGTVGDLAG LQNGSDLQFIEMQRDANRQI SEYKFKLSKAEQDITTLEQS ISRLEGQVLRY
Tissue Specificity Widely expressed, with highest levels in heart and skeletal muscle.
Post Translational Modifications Ser-190 and Ser-202 are phosphorylated in response to LPS stimulation. Ser-202 phosphorylation regulates the LPS-induced interaction with MYD88.
Function May function as activator of the canonical Wnt signaling pathway, in association with DVL3, upstream of CTNNB1/beta-catenin. Positively regulates Toll-like receptor (TLR) signaling in response to agonist probably by competing with the negative FLII regulator for MYD88-binding.
Protein Name Leucine-Rich Repeat Flightless-Interacting Protein 2
Lrr Flii-Interacting Protein 2
Alternative Antibody Names Anti-Leucine-Rich Repeat Flightless-Interacting Protein 2 antibody
Anti-Lrr Flii-Interacting Protein 2 antibody
Anti-LRRFIP2 antibody

Information sourced from Uniprot.org

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