• Western blot analysis of various lysates, using LRP5 Rabbit monoclonal antibody (STJ11104803) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-LRP5 antibody (1400-1500) [S4803RM] (STJ11104803)

SKU:
STJ11104803

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Low-Density Lipoprotein Receptor-Related Protein 5 (1400-1500) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S4803RM
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LRP5
Gene ID: 4041
Uniprot ID: LRP5_HUMAN
Immunogen Region: 1400-1500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1400-1500 of human LRP5 (NP_002326.2).
Immunogen Sequence: MGGVYFVCQRVVCQRYAGAN GPFPHEYVSGTPHVPLNFIA PGGSQHGPFTGIACGKSMMS SVSLMGGRGGVPLYDRNHVT GASSSSSSSTKATLYPPILN P
Tissue Specificity Widely expressed, with the highest level of expression in the liver and in aorta.
Post Translational Modifications Phosphorylation of cytoplasmic PPPSP motifs regulates the signal transduction of the Wnt signaling pathway through acting as a docking site for AXIN1.
Function Acts as a coreceptor with members of the frizzled family of seven-transmembrane spanning receptors to transduce signal by Wnt proteins. Activates the canonical Wnt signaling pathway that controls cell fate determination and self-renewal during embryonic development and adult tissue regeneration. In particular, may play an important role in the development of the posterior patterning of the epiblast during gastrulation. During bone development, regulates osteoblast proliferation and differentiation thus determining bone mass. Mechanistically, the formation of the signaling complex between Wnt ligand, frizzled receptor and LRP5 coreceptor promotes the recruitment of AXIN1 to LRP5, stabilizing beta-catenin/CTNNB1 and activating TCF/LEF-mediated transcriptional programs. Acts as a coreceptor for non-Wnt proteins, such as norrin/NDP. Binding of norrin/NDP to frizzled 4/FZD4-LRP5 receptor complex triggers beta-catenin/CTNNB1-dependent signaling known to be required for retinal vascular development. Plays a role in controlling postnatal vascular regression in retina via macrophage-induced endothelial cell apoptosis.
Protein Name Low-Density Lipoprotein Receptor-Related Protein 5
Lrp-5
Low-Density Lipoprotein Receptor-Related Protein 7
Lrp-7
Database Links Reactome: R-HSA-201681
Reactome: R-HSA-3772470
Reactome: R-HSA-4641262
Reactome: R-HSA-4641263
Reactome: R-HSA-5339717
Reactome: R-HSA-5340588
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Endoplasmic Reticulum
Chaperoned To The Plasma Membrane By Mesd
Alternative Antibody Names Anti-Low-Density Lipoprotein Receptor-Related Protein 5 antibody
Anti-Lrp-5 antibody
Anti-Low-Density Lipoprotein Receptor-Related Protein 7 antibody
Anti-Lrp-7 antibody
Anti-LRP5 antibody
Anti-LR3 antibody
Anti-LRP7 antibody

Information sourced from Uniprot.org

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