Anti-LPL antibody (124-475) (STJ118691)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LPL (124-475) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LPL |
| Gene ID | 4023 |
| Uniprot ID | LIPL_HUMAN |
| Immunogen Region | 124-475 |
| Immunogen Sequence | SAGYTKLVGQDVARFINWME EEFNYPLDNVHLLGYSLGAH AAGIAGSLTNKKVNRITGLD PAGPNFEYAEAPSRLSPDDA DFVDVLHTFTRGSPGRSIGI QKPVGHVDIYPNGGTFQPGC NIGEAIRVIAERGLGDVDQL VKCSHERSIHLFIDSLLNEE NPSKAYRCSSKEAFEKGLCL SCRKNRCNNLGYEINKVRAK RSSKMYLKTRSQMPYKVFHY QVKIHFSGTESETHTNQAF |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 124-475 of human Lipoprotein lipase (Lipoprotein lipase (LPL) ) (NP_000228.1). |
Additional Info
| Tissue Specificity | Detected in blood plasma. Detected in milk (at protein level). |
| Post Translational Modifications | Tyrosine nitration after lipopolysaccharide (LPS) challenge down-regulates the lipase activity. |
| Function | Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Although it has both phospholipase and triglyceride lipase activities it is primarily a triglyceride lipase with low but detectable phospholipase activity. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. |
| Protein Name | Lipoprotein LipaseLplPhospholipase A1 |
| Database Links | Reactome: R-HSA-381340Reactome: R-HSA-8963889Reactome: R-HSA-8963901Reactome: R-HSA-975634Reactome: R-HSA-9841922 |
| Cellular Localisation | Cell MembranePeripheral Membrane ProteinExtracellular SideSecretedExtracellular SpaceExtracellular MatrixNewly Synthesized Lpl Binds To Cell Surface Heparan Proteoglycans And Is Then Released By HeparanaseSubsequentlyIt Becomes Attached To Heparan Proteoglycan On Endothelial CellsLocates To The Plasma Membrane Of Microvilli Of Hepatocytes With Triglyceride-Rich Lipoproteins (Trl)Some Of The Bound Lpl Is Then Internalized And Located Inside Non-Coated Endocytic Vesicles |
| Alternative Antibody Names | Anti-Lipoprotein Lipase antibodyAnti-Lpl antibodyAnti-Phospholipase A1 antibodyAnti-LPL antibodyAnti-LIPD antibody |
Information sourced from Uniprot.org