Anti-LONP1 antibody (120-400) (STJ24417)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal LONP1 (120-400) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LONP1 |
| Gene ID | 9361 |
| Uniprot ID | LONM_HUMAN |
| Immunogen Region | 120-400 |
| Immunogen Sequence | VFPHLPLIAITRNPVFPRFI KIIEVKNKKLVELLRRKVRL AQPYVGVFLKRDDSNESDVV ESLDEIYHTGTFAQIHEMQD LGDKLRMIVMGHRRVHISRQ LEVEPEEPEAENKHKPRRKS KRGKKEAEDELSARHPAELA MEPTPELPAEVLMVEVENVV HEDFQVTEEVKALTAEIVKT IRDIIALNPLYRESVLQMMQ AGQRVVDNPIYLSDMGAALT GAESHELQDVLEETNIPKR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 120-400 of human LONP1 (NP_004784.2). |
Additional Info
| Tissue Specificity | Duodenum, heart, lung and liver, but not thymus. |
| Function | ATP-dependent serine protease that mediates the selective degradation of misfolded, unassembled or oxidatively damaged polypeptides as well as certain short-lived regulatory proteins in the mitochondrial matrix. Endogenous substrates include mitochondrial steroidogenic acute regulatory (StAR) protein, DELE1, helicase Twinkle (TWNK) and the large ribosomal subunit protein MRPL32/bL32m. MRPL32/bL32m is protected from degradation by LONP1 when it is bound to a nucleic acid (RNA), but TWNK is not. May also have a chaperone function in the assembly of inner membrane protein complexes. Participates in the regulation of mitochondrial gene expression and in the maintenance of the integrity of the mitochondrial genome. Binds to mitochondrial promoters and RNA in a single-stranded, site-specific, and strand-specific manner. May regulate mitochondrial DNA replication and/or gene expression using site-specific, single-stranded DNA binding to target the degradation of regulatory proteins binding to adjacent sites in mitochondrial promoters. |
| Protein Name | Lon Protease Homolog - MitochondrialLonhsLon Protease-Like ProteinLonpMitochondrial Atp-Dependent Protease LonSerine Protease 15 |
| Database Links | Reactome: R-HSA-9837999Reactome: R-HSA-9841251 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Lon Protease Homolog - Mitochondrial antibodyAnti-Lonhs antibodyAnti-Lon Protease-Like Protein antibodyAnti-Lonp antibodyAnti-Mitochondrial Atp-Dependent Protease Lon antibodyAnti-Serine Protease 15 antibodyAnti-LONP1 antibodyAnti-PRSS15 antibody |
Information sourced from Uniprot.org