| Host: |
Mouse |
| Applications: |
WB/IHC/IF/IP |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-Lamin-B1 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
7C11 |
| Conjugation: |
Cy5 |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS pH7.4, containing 0.02% Sodium Azide and 50% Glycerol. |
| Purification: |
The antibody was purified using affinity-chromatography using specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
Optimal working dilutions should be determined experimentally by the investigator Suggested starting dilutions are as follows: IHC 1:50-300 IF 1:200 |
| Storage Instruction: |
Stable for one year at-15°C to-25°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezi |
| Gene Symbol: |
LMNB1 |
| Gene ID: |
4001 |
| Uniprot ID: |
LMNB1_HUMAN |
| Specificity: |
Lamin B1 Monoclonal Antibody (7C11) Cy5 Conjugated specially designed for your Immunofluorescence analysis. |
| Post Translational Modifications | B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation plays a key role in lamin organization, subcellular localization and nuclear envelope disintegration. Phosphorylation by CDK1 at Ser-23 and Ser-393 at the onset of mitosis drives lamin disassembly and nuclear envelope breakdown. |
| Function | Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. |
| Protein Name | Lamin-B1 |
| Database Links | Reactome: R-HSA-1221632Reactome: R-HSA-2559584Reactome: R-HSA-2980766Reactome: R-HSA-2995383Reactome: R-HSA-352238Reactome: R-HSA-4419969Reactome: R-HSA-8862803Reactome: R-HSA-8950505Reactome: R-HSA-9013405Reactome: R-HSA-9035034 |
| Cellular Localisation | Nucleus Lamina |
| Alternative Antibody Names | Anti-Lamin-B1 antibodyAnti-LMNB1 antibodyAnti-LMN2 antibodyAnti-LMNB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
