• Western blot analysis of lysates from NIH/3T3 cells, treated with PMA 125ng/ml 30', using LIMK2 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of the lysates from RAW264.7cells using LIMK2 antibody.
  • Western blot analysis of 3T3 cells using LIMK-2 Polyclonal Antibody

Anti-LIMK2 antibody (249-298 aa) (STJ93931)

SKU:
STJ93931

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LIM domain kinase 2 (249-298 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LIMK2
Gene ID: 3985
Uniprot ID: LIMK2_HUMAN
Immunogen Region: 249-298 aa
Specificity: LIMK-2 Polyclonal Antibody detects endogenous levels of LIMK-2 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human LIMK2 at the amino acid range 249-298
Function Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stages of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. Suppresses ciliogenesis via multiple pathways.phosphorylation of CFL1, suppression of directional trafficking of ciliary vesicles to the ciliary base, and by facilitating YAP1 nuclear localization where it acts as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1.
Protein Name Lim Domain Kinase 2
Limk-2
Database Links Reactome: R-HSA-3928662
Reactome: R-HSA-416572
Reactome: R-HSA-5627117
Cellular Localisation Cytoplasm
Cytoskeleton
Spindle
Microtubule Organizing Center
Centrosome
Isoform Limk2a: Cytoplasm
Nucleus
Isoform Limk2b: Cytoplasm
Perinuclear Region
Mainly Present In The Cytoplasm And Is Scarcely Translocated To The Nucleus
Alternative Antibody Names Anti-Lim Domain Kinase 2 antibody
Anti-Limk-2 antibody
Anti-LIMK2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance