Anti-LIMD1 antibody (STJA0008711)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human LIMD1
General Information
| Short Description | Rabbit polyclonal anti-LIM domain-containing protein 1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LIMD1 |
| Gene ID | 8994 |
| Uniprot ID | LIMD1_HUMAN |
| Immunogen | Synthesized peptide derived from human LIMD1 |
| Specificity | This antibody detects endogenous levels of LIMD1 at Human, Mouse, Rat |
Additional Info
| Post Translational Modifications | Phosphorylated during mitosis. |
| Function | Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG-dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. |
| Protein Name | Lim Domain-Containing Protein 1 |
| Database Links | Reactome: R-HSA-1234176 |
| Cellular Localisation | CytoplasmNucleusP-BodyCell JunctionAdherens JunctionFocal AdhesionShuttles Between Cytoplasm And Nucleus But Is Localized Predominantly To The CytoplasmFound In The Nucleus But Not NucleoliColocalizes With Vcl In The Focal AdhesionsDown-Regulation And/Or Elimination Of Its Expression From The Nucleus Of Neoplastic Cells Correlates Strongly With Poor Patient Prognosis And Aggressive Forms Of Breast CarcinomaConverselyStrong Nuclear Localization Correlates With Low-Tumor Grade And Better Patient Prognosis |
| Alternative Antibody Names | Anti-Lim Domain-Containing Protein 1 antibodyAnti-LIMD1 antibody |
Information sourced from Uniprot.org