| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : |
Goat polyclonal antibody anti-LIG1/DNA ligase I (Internal) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 125kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 102kDa according to NP_000225.1). This molecular weight is routinely observed by other sources. Recom |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
LIG1 |
| Gene ID: |
3978 |
| Uniprot ID: |
DNLI1_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_000225.1 |
| Immunogen Sequence: |
RVREDKQPEQATTS |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/185995/407755/STJ11106799_1__67131.1744894266.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and DNA Ligase IV knockout (KO) HeLa cells, using [KO Validated] DNA Ligase IV Rabbit pAb (STJ11106799) at 1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 1min.")
