• Western blot analysis of lysates from HeLa cells, using Galectin 3 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using CBP 35 Polyclonal Antibody diluted at 1:2000
  • Immunofluorescence analysis of NIH/3T3 cells, using Galectin 3 Antibody. The picture on the right is blocked with the synthesized peptide.
  • Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS3 antibody (141-190 aa) (STJ92060)

SKU:
STJ92060

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Galectin-3 (141-190 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LGALS3
Gene ID: 3958
Uniprot ID: LEG3_HUMAN
Immunogen Region: 141-190 aa
Specificity: CBP 35 Polyclonal Antibody detects endogenous levels of CBP 35 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Galectin 3 at the amino acid range 141-190
Function Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Protein Name Galectin-3
Gal-3
35 Kda Lectin
Carbohydrate-Binding Protein 35
Cbp 35
Galactose-Specific Lectin 3
Galactoside-Binding Protein
Galbp
Ige-Binding Protein
L-31
Laminin-Binding Protein
Lectin L-29
Mac-2 Antigen
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-879415
Reactome: R-HSA-8939246
Reactome: R-HSA-8941333
Cellular Localisation Cytoplasm
Nucleus
Secreted
Secreted By A Non-Classical Secretory Pathway And Associates With The Cell Surface
Can Be Secreted
The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10
It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative Antibody Names Anti-Galectin-3 antibody
Anti-Gal-3 antibody
Anti-35 Kda Lectin antibody
Anti-Carbohydrate-Binding Protein 35 antibody
Anti-Cbp 35 antibody
Anti-Galactose-Specific Lectin 3 antibody
Anti-Galactoside-Binding Protein antibody
Anti-Galbp antibody
Anti-Ige-Binding Protein antibody
Anti-L-31 antibody
Anti-Laminin-Binding Protein antibody
Anti-Lectin L-29 antibody
Anti-Mac-2 Antigen antibody
Anti-LGALS3 antibody
Anti-MAC2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance