• STJ70931 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70931 (1µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70931 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Leptin Receptor antibody (Internal) (STJ70931)

SKU:
STJ70931-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC/FC
Reactivity: Human/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Leptin Receptor (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-4µg/ml
FC-Flow cytometric analysis of K562 cells. 10ug/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: LEPR
Gene ID: 3953
Uniprot ID: LEPR_HUMAN
Immunogen Region: Internal
Accession Number: NP_002294.2; NP_001003679.1; NP_001003680.1
Specificity: This antibody is expected to recognise all three reported isoforms (NP_002294.2; NP_001003679.1; NP_001003680.1).
Immunogen Sequence: TQDDIEKHQSDAG
Post Translational Modifications On ligand binding, phosphorylated on two conserved C-terminal tyrosine residues (isoform B only) by JAK2. Tyr-986 is required for complete binding and activation of PTPN11, ERK/FOS activation,for interaction with SOCS3 and SOCS3 mediated inhibition of leptin signaling. Phosphorylation on Tyr-1141 is required for STAT3 binding/activation. Phosphorylation of Tyr-1079 has a more accessory role.
Function Receptor for hormone LEP/leptin (Probable). On ligand binding, mediates LEP central and peripheral effects through the activation of different signaling pathways such as JAK2/STAT3 and MAPK cascade/FOS. In the hypothalamus, LEP acts as an appetite-regulating factor that induces a decrease in food intake and an increase in energy consumption by inducing anorexinogenic factors and suppressing orexigenic neuropeptides, also regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones. In the periphery, increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, is pro-angiogenic and affects innate and adaptive immunity. Control of energy homeostasis and melanocortin production (stimulation of POMC and full repression of AgRP transcription) is mediated by STAT3 signaling, whereas distinct signals regulate NPY and the control of fertility, growth and glucose homeostasis. Involved in the regulation of counter-regulatory response to hypoglycemia by inhibiting neurons of the parabrachial nucleus. Has a specific effect on T lymphocyte responses, differentially regulating the proliferation of naive and memory T -ells. Leptin increases Th1 and suppresses Th2 cytokine production. Isoform A: May transport LEP across the blood-brain barrier. Binds LEP and mediates LEP endocytosis. Does not induce phosphorylation of and activate STAT3. Isoform E: Antagonizes Isoform A and isoform B-mediated LEP binding and endocytosis.
Protein Name Leptin Receptor
Lep-R
Hub219
Ob Receptor
Ob-R
Cd Antigen Cd295
Database Links Reactome: R-HSA-2586552 P48357-1
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Basolateral Cell Membrane
Isoform E: Secreted
Alternative Antibody Names Anti-Leptin Receptor antibody
Anti-Lep-R antibody
Anti-Hub219 antibody
Anti-Ob Receptor antibody
Anti-Ob-R antibody
Anti-Cd Antigen Cd295 antibody
Anti-LEPR antibody
Anti-DB antibody
Anti-OBR antibody

Information sourced from Uniprot.org

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