Anti-Legumain (LGMN) antibody [SRM14679] (STJ11109409)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenRecombinant protein (or fragment).This information is considered to be commercially sensitive.
General Information
| Short Description | Rabbit monoclonal Legumain (LGMN) antibody for use in WB, IF-P, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF-P/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | SRM14679 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | WB, 1:1000-1:6000IF-P, 1:50-1:200IHC-P, 1:1000-1:5000ELISA, Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | LGMN |
| Gene ID | 5641 |
| Uniprot ID | LGMN_HUMAN |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
Additional Info
| Tissue Specificity | Ubiquitous. Particularly abundant in kidney, heart and placenta. |
| Post Translational Modifications | Activated by autocatalytic processing at pH 4. |
| Function | Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Also involved in MHC class I antigen presentation in cross-presenting dendritic cells by mediating cleavage and maturation of Perforin-2 (MPEG1), thereby promoting antigen translocation in the cytosol. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation. |
| Protein Name | LegumainAsparaginyl EndopeptidaseAepProtease - Cysteine 1 |
| Database Links | Reactome: R-HSA-1679131Reactome: R-HSA-196791Reactome: R-HSA-2132295 |
| Cellular Localisation | Lysosome |
| Alternative Antibody Names | Anti-Legumain antibodyAnti-Asparaginyl Endopeptidase antibodyAnti-Aep antibodyAnti-Protease - Cysteine 1 antibodyAnti-LGMN antibodyAnti-PRSC1 antibody |
Information sourced from Uniprot.org