• STJ70898 (2µg/ml) staining of K562 nuclear cell lysate (A) + peptide (B). (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70898 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70898 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70898 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-LEF1 antibody (Internal) (STJ70898)

SKU:
STJ70898-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LEF1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei of Jurkat and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: LEF1
Gene ID: 51176
Uniprot ID: LEF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_057353.1; NP_001124185.1; NP_001124186.1; NP_001159591.1
Immunogen Sequence: QHEQRKEQEPKRPH
Post Translational Modifications Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway.
Function Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling. May play a role in hair cell differentiation and follicle morphogenesis. Isoform 1: Transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Isoform 3: Lacks the CTNNB1 interaction domain and may therefore be an antagonist for Wnt signaling. Isoform 5: Transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells.
Protein Name Lymphoid Enhancer-Binding Factor 1
Lef-1
T Cell-Specific Transcription Factor 1-Alpha
Tcf1-Alpha
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-3769402
Reactome: R-HSA-4086398
Reactome: R-HSA-4411364
Reactome: R-HSA-4641265
Reactome: R-HSA-8853884
Reactome: R-HSA-8951430
Reactome: R-HSA-9616222
Reactome: R-HSA-9733709
Reactome: R-HSA-9754189
Reactome: R-HSA-9793380
Reactome: R-HSA-9796292
Reactome: R-HSA-9824272
Cellular Localisation Nucleus
Found In Nuclear Bodies Upon Piasg Binding
Alternative Antibody Names Anti-Lymphoid Enhancer-Binding Factor 1 antibody
Anti-Lef-1 antibody
Anti-T Cell-Specific Transcription Factor 1-Alpha antibody
Anti-Tcf1-Alpha antibody
Anti-LEF1 antibody

Information sourced from Uniprot.org

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