• Immunofluorescence analysis of NIH/3T3 cells using LDL Receptor (LDLR) Rabbit polyclonal antibody (STJ11104555) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Rat kidney using LDL Receptor (LDLR) antibody (STJ11104555) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Mouse lung using LDL Receptor (LDLR) antibody (STJ11104555) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-LDLR antibody (761-860) (STJ11104555)

SKU:
STJ11104555

£112.50 - £524.50
Current Stock:
Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LDLR
Gene ID: 3949
Uniprot ID: LDLR_HUMAN
Immunogen Region: 761-860
Specificity: A synthetic peptide corresponding to a sequence within amino acids 761-860 of human LDL Receptor (LDLR) (NP_000518.1).
Immunogen Sequence: TVEIVTMSHQALGDVAGRGN EKKPSSVRALSIVLPIVLLV FLCLGVFLLWKNWRLKNINS INFDNPVYQKTTEDEVHICH NQDGYSYPSRQMVSLEDDVA
Post Translational Modifications N- and O-glycosylated. Ubiquitinated by MYLIP leading to degradation.
Function Binds low density lipoprotein /LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Forms a ternary complex with PGRMC1 and TMEM97 receptors which increases LDLR-mediated LDL internalization. (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes, but not through a direct interaction with viral proteins. (Microbial infection) Acts as a receptor for Vesicular stomatitis virus. (Microbial infection) In case of HIV-1 infection, may function as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. (Microbial infection) Acts as a receptor for Crimean-Congo hemorrhagic fever virus (CCHFV). (Microbial infection) Acts as a receptor for many Alphavirus, including Getah virus (GETV), Ross river virus (RRV) and Semliki Forest virus.
Protein Name Low-Density Lipoprotein Receptor
Ldl Receptor
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-8964026
Reactome: R-HSA-8964038
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane
Clathrin-Coated Pit
Golgi Apparatus
Early Endosome
Late Endosome
Lysosome
Rapidly Endocytosed Upon Ligand Binding
Localized At Cell Membrane
Probably In Lipid Rafts
In Serum-Starved Conditions
Alternative Antibody Names Anti-Low-Density Lipoprotein Receptor antibody
Anti-Ldl Receptor antibody
Anti-LDLR antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance