| Host: | Camel |
| Applications: | FC/ELISA |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Camel monoclonal antibody anti-Neutrophil gelatinase-associated lipocalin is suitable for use in Flow Cytometry and ELISA research applications. |
| Clonality : | Monoclonal |
| Conjugation: | Unconjugated |
| Formulation: | Liquid in PBS |
| Purification: | The antibody was affinity-purified nickel column using his-tag in antibody |
| Concentration: | 1 mg/mL |
| Dilution Range: | ELISA 1:10000-20000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LCN2 |
| Gene ID: | 3934 |
| Uniprot ID: | NGAL_HUMAN |
| Specificity: | This antibody detects endogenous levels of NGAL. |
| Immunogen: | Recombinant protein of full lenth human NGAL. |
| Tissue Specificity | Detected in neutrophils (at protein level). Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Expressed in the medullary tubules of the kidney. Not found in the small intestine or peripheral blood leukocytes. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated Lipocalin Ngal 25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9 Lipocalin-2 Oncogene 24p3 Siderocalin P25 |
| Database Links | Reactome: R-HSA-6785807 Reactome: R-HSA-6798695 Reactome: R-HSA-6799990 Reactome: R-HSA-917937 |
| Cellular Localisation | Secreted Cytoplasmic Granule Lumen Cytoplasmic Vesicle Lumen Upon Binding To The Slc22a17 (24p3r) Receptor It Is Internalized Releases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Antibody Names | Anti-Neutrophil Gelatinase-Associated Lipocalin antibody Anti-Ngal antibody Anti-25 Kda Alpha-2-Microglobulin-Related Subunit Of Mmp-9 antibody Anti-Lipocalin-2 antibody Anti-Oncogene 24p3 antibody Anti-Siderocalin antibody Anti-P25 antibody Anti-LCN2 antibody Anti-HNL antibody Anti-NGAL antibody |
Information sourced from Uniprot.org