Anti-LCAT antibody (366-378) (STJ73565)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-LCAT (366-378) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Pig/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:1000.WB: Approx 55kDa band observed in Human Testis lysates (calculated MW of 49.6kDa according to NP_000220.1). This molecular weight is routinely observed by other sources. Recommended concent |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20ยฐC on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | LCAT |
| Gene ID | 3931 |
| Uniprot ID | LCAT_HUMAN |
| Accession Number | NP_000220.1 |
| Immunogen Region | 366-378 |
| Immunogen Sequence | EDGDDTVATRSTE |
Additional Info
| Post Translational Modifications | O- and N-glycosylated. O-glycosylation on Thr-431 and Ser-433 consists of sialylated galactose beta 1-->3N-acetylgalactosamine structures. N-glycosylated sites contain sialylated triantennary and/or biantennary complex structures. |
| Function | Central enzyme in the extracellular metabolism of plasma lipoproteins. Synthesized mainly in the liver and secreted into plasma where it converts cholesterol and phosphatidylcholines (lecithins) to cholesteryl esters and lysophosphatidylcholines on the surface of high and low density lipoproteins (HDLs and LDLs). The cholesterol ester is then transported back to the liver. Has a preference for plasma 16:0-18:2 or 18:O-18:2 phosphatidylcholines. Also produced in the brain by primary astrocytes, and esterifies free cholesterol on nascent APOE-containing lipoproteins secreted from glia and influences cerebral spinal fluid (CSF) APOE- and APOA1 levels. Together with APOE and the cholesterol transporter ABCA1, plays a key role in the maturation of glial-derived, nascent lipoproteins. Required for remodeling high-density lipoprotein particles into their spherical forms. Catalyzes the hydrolysis of 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor or PAF) to 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). Also catalyzes the transfer of the acetate group from PAF to 1-hexadecanoyl-sn-glycero-3-phosphocholine forming lyso-PAF. Catalyzes the esterification of (24S)-hydroxycholesterol (24(S)OH-C), also known as cerebrosterol to produce 24(S)OH-C monoesters. |
| Protein Name | Phosphatidylcholine-Sterol Acyltransferase1-Alkyl-2-Acetylglycerophosphocholine EsteraseLecithin-Cholesterol AcyltransferasePhospholipid-Cholesterol AcyltransferasePlatelet-Activating Factor AcetylhydrolasePaf Acetylhydrolase |
| Database Links | Reactome: R-HSA-8964058 |
| Cellular Localisation | SecretedSecreted Into Blood PlasmaProduced In Astrocytes And Secreted Into Cerebral Spinal Fluid (Csf) |
| Alternative Antibody Names | Anti-Phosphatidylcholine-Sterol Acyltransferase antibodyAnti-1-Alkyl-2-Acetylglycerophosphocholine Esterase antibodyAnti-Lecithin-Cholesterol Acyltransferase antibodyAnti-Phospholipid-Cholesterol Acyltransferase antibodyAnti-Platelet-Activating Factor Acetylhydrolase antibodyAnti-Paf Acetylhydrolase antibodyAnti-LCAT antibody |
Information sourced from Uniprot.org