• STJ70565 (0. 01µg/ml) staining of Peripheral Blood Mononucleocytes lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-LAT1 antibody (C-Term) (STJ70565)

SKU:
STJ70565-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-LAT1 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:2000.
WB: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 27.9kDa according to NP_055202.1). This molecular weight is routinely observed by other so
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: LAT
Gene ID: 27040
Uniprot ID: LAT_HUMAN
Immunogen Region: C-Term
Accession Number: NP_055202.1; NP_001014987.1; NP_001014989.1; NP_001014988.1
Specificity: This antibody is expected to recognize isoform a (NP_055202.1) , isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1).
Immunogen Sequence: GAPDYENLQELN
Post Translational Modifications Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation.which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes. Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. 'Lys-63'-linked ubiquitinated by TRAF6.
Function Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.
Protein Name Linker For Activation Of T-Cells Family Member 1
36 Kda Phosphotyrosine Adapter Protein
Pp36
P36-38
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-202433
Reactome: R-HSA-2424491 O43561-2
Reactome: R-HSA-2454202 O43561-2
Reactome: R-HSA-2871796 O43561-2
Reactome: R-HSA-2871809 O43561-2
Reactome: R-HSA-5673001 O43561-2
Cellular Localisation Cell Membrane
Single-Pass Type Iii Membrane Protein
Present In Lipid Rafts
Alternative Antibody Names Anti-Linker For Activation Of T-Cells Family Member 1 antibody
Anti-36 Kda Phosphotyrosine Adapter Protein antibody
Anti-Pp36 antibody
Anti-P36-38 antibody
Anti-LAT antibody

Information sourced from Uniprot.org

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