• Immunofluorescence analysis of BALA-3T3 cells using LAMP1 Rabbit polyclonal antibody (STJ119222) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Human lung tissue using LAMP1 Rabbit polyclonal antibody (STJ119222) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human colon tissue using LAMP1 Rabbit polyclonal antibody (STJ119222) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using LAMP1 Rabbit polyclonal antibody (STJ119222) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using LAMP1 Rabbit polyclonal antibody (STJ119222) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-LAMP1 antibody (185-202) (STJ119222)

SKU:
STJ119222

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Host: Rabbit
Applications: WB/IF/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-LAMP1 (185-202) is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: LAMP1
Gene ID: 3916
Uniprot ID: LAMP1_HUMAN
Immunogen Region: 185-202
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 185-202 of human LAMP1 (NP_005552.3).
Immunogen Sequence: SRGETRCEQDRPSPTTAP
Post Translational Modifications O- and N-glycosylated.some of the 18 N-linked glycans are polylactosaminoglycans. (Microbial infection) The glycosylation of Asn-76 is essential for Lassa virus entry into cells.
Function Lysosomal membrane glycoprotein which plays an important role in lysosome biogenesis, lysosomal pH regulation, autophagy and cholesterol homeostasis. Acts as an important regulator of lysosomal lumen pH regulation by acting as a direct inhibitor of the proton channel TMEM175, facilitating lysosomal acidification for optimal hydrolase activity. Also plays an important role in NK-cells cytotoxicity. Mechanistically, participates in cytotoxic granule movement to the cell surface and perforin trafficking to the lytic granule. In addition, protects NK-cells from degranulation-associated damage induced by their own cytotoxic granule content. Presents carbohydrate ligands to selectins. (Microbial infection) Acts as a receptor for Lassa virus glycoprotein. Promotes also fusion of the virus with host membrane in less acidic endosomes. (Microbial infection) Supports the FURIN-mediated cleavage of mumps virus fusion protein F by interacting with both FURIN and the unprocessed form but not the processed form of the viral protein F.
Protein Name Lysosome-Associated Membrane Glycoprotein 1
Lamp-1
Lysosome-Associated Membrane Protein 1
Cd107 Antigen-Like Family Member A
Cd Antigen Cd107a
Database Links Reactome: R-HSA-6798695
Cellular Localisation Lysosome Membrane
Single-Pass Type I Membrane Protein
Endosome Membrane
Late Endosome Membrane
Cell Membrane
Cytolytic Granule Membrane
This Protein Shuttles Between Lysosomes
Endosomes
And The Plasma Membrane
Colocalizes With Osbpl1a At The Late Endosome
Alternative Antibody Names Anti-Lysosome-Associated Membrane Glycoprotein 1 antibody
Anti-Lamp-1 antibody
Anti-Lysosome-Associated Membrane Protein 1 antibody
Anti-Cd107 Antigen-Like Family Member A antibody
Anti-Cd Antigen Cd107a antibody
Anti-LAMP1 antibody

Information sourced from Uniprot.org

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