| Short Description : | Rabbit polyclonal LAMB1 (1467-1786) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:4000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | LAMB1 |
| Gene ID: | 3912 |
| Uniprot ID: | LAMB1_HUMAN |
| Immunogen Region: | 1467-1786 |
| Immunogen Sequence: | EAKLRADEAKQSAEDILLKT NATKEKMDKSNEELRNLIKQ IRNFLTQDSADLDSIEAVAN EVLKMEMPSTPQQLQNLTED IRERVESLSQVEVILQHSAA DIARAEMLLEEAKRASKSAT DVKVTADMVKEALEEAEKAQ VAAEKAIKQADEDIQGTQNL LTSIESETAASEETLFNASQ RISELERNVEELKRKAAQNS GEAEYIEKVVYTVKQSAEDV KKTLDGELDEKYKKVENLI |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1467-1786 of human Laminin beta 1 (NP_002282.2). |
| Function | Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. Involved in the organization of the laminar architecture of cerebral cortex. It is probably required for the integrity of the basement membrane/glia limitans that serves as an anchor point for the endfeet of radial glial cells and as a physical barrier to migrating neurons. Radial glial cells play a central role in cerebral cortical development, where they act both as the proliferative unit of the cerebral cortex and a scaffold for neurons migrating toward the pial surface. |
| Protein Name | Laminin Subunit Beta-1Laminin B1 ChainLaminin-1 Subunit BetaLaminin-10 Subunit BetaLaminin-12 Subunit BetaLaminin-2 Subunit BetaLaminin-6 Subunit BetaLaminin-8 Subunit Beta |
| Database Links | Reactome: R-HSA-1474228Reactome: R-HSA-3000157Reactome: R-HSA-3000171Reactome: R-HSA-3000178Reactome: R-HSA-373760Reactome: R-HSA-381426Reactome: R-HSA-8874081Reactome: R-HSA-8957275Reactome: R-HSA-9619665Reactome: R-HSA-9913351 |
| Cellular Localisation | SecretedExtracellular SpaceExtracellular MatrixBasement MembraneMajor Component |
| Alternative Antibody Names | Anti-Laminin Subunit Beta-1 antibodyAnti-Laminin B1 Chain antibodyAnti-Laminin-1 Subunit Beta antibodyAnti-Laminin-10 Subunit Beta antibodyAnti-Laminin-12 Subunit Beta antibodyAnti-Laminin-2 Subunit Beta antibodyAnti-Laminin-6 Subunit Beta antibodyAnti-Laminin-8 Subunit Beta antibodyAnti-LAMB1 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit polyclonal antibody (STJ11100846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/89780/358251/STJ11100846_1__65438.1713122587.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit polyclonal antibody (STJ11100846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit polyclonal antibody (STJ11100846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/89780/358251/STJ11100846_1__65438.1713122587.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Laminin beta 1 knockout (KO) HeLa cells, using [KO Validated] Laminin beta 1 Rabbit polyclonal antibody (STJ11100846) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
