• Immunofluorescence analysis of C6 cells using KSR1 Rabbit polyclonal antibody (STJ117312) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using KSR1 Rabbit polyclonal antibody (STJ117312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-KSR1 antibody (320-420) (STJ117312)

SKU:
STJ117312

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KSR1 (320-420) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KSR1
Gene ID: 8844
Uniprot ID: KSR1_HUMAN
Immunogen Region: 320-420
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 320-420 of human KSR1 (NP_055053.1).
Immunogen Sequence: SSPAPFPTSSNPSSATTPPN PSPGQRDSRFNFPAAYFIHH RQQFIFPVPSAGHCWKCLLI AESLKENAFNISAFAHAAPL PEAADGTRLDDQPKADVLEA H
Post Translational Modifications Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3.
Function Part of a multiprotein signaling complex which promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Independently of its kinase activity, acts as MAP2K1/MEK1 and MAP2K2/MEK2-dependent allosteric activator of BRAF.upon binding to MAP2K1/MEK1 or MAP2K2/MEK2, dimerizes with BRAF and promotes BRAF-mediated phosphorylation of MAP2K1/MEK1 and/or MAP2K2/MEK2. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Its kinase activity is unsure. Some protein kinase activity has been detected in vitro, however the physiological relevance of this activity is unknown.
Protein Name Kinase Suppressor Of Ras 1
Database Links Reactome: R-HSA-5673000
Reactome: R-HSA-5674135
Reactome: R-HSA-5675221
Reactome: R-HSA-6802946
Reactome: R-HSA-6802948
Reactome: R-HSA-6802952
Reactome: R-HSA-6802955
Reactome: R-HSA-9649948
Reactome: R-HSA-9656223
Cellular Localisation Cytoplasm
Membrane
Peripheral Membrane Protein
Cell Membrane
Cell Projection
Ruffle Membrane
Endoplasmic Reticulum Membrane
In Unstimulated Cells
Where The Phosphorylated Form Is Bound To A 14-3-3 Protein
Sequestration In The Cytoplasm Occurs
Following Growth Factor Treatment
The Protein Is Free For Membrane Translocation
And It Moves From The Cytoplasm To The Cell Periphery
Alternative Antibody Names Anti-Kinase Suppressor Of Ras 1 antibody
Anti-KSR1 antibody
Anti-KSR antibody

Information sourced from Uniprot.org

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