• SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK20 (ABT044) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: SW480Predicted band size: 48kDaObserved band size: 48kDa
  • Human appendix tissue was stained with Anti-Cytokeratin 20 (ABT044) Antibody
  • Human colon carcinoma tissue was stained with Anti-Cytokeratin 20 (ABT044) Antibody
  • Human rectal carcinoma tissue was stained with Anti-Cytokeratin 20 (ABT044) Antibody

Anti-KRT20 antibody (300-424 aa) [ABT044] (STJ197146)

SKU:
STJ197146

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type I cytoskeletal 20 (300-424 aa) is suitable for use in Immunohistochemistry, Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT044
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF 1:100-500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT20
Gene ID: 54474
Uniprot ID: K1C20_HUMAN
Immunogen Region: 300-424 aa
Specificity: The antibody can specifically recognize human CK20 protein, and shows no cross reaction with CK1, 5, 6, 7, 8, 13, 14, 15, 17, 18.
Immunogen: Synthesized peptide derived from the human CK20 at the amino acid range 300-424
Post Translational Modifications Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Protein Name Keratin - Type I Cytoskeletal 20
Cytokeratin-20
Ck-20
Keratin-20
K20
Protein It
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 20 antibody
Anti-Cytokeratin-20 antibody
Anti-Ck-20 antibody
Anti-Keratin-20 antibody
Anti-K20 antibody
Anti-Protein It antibody
Anti-KRT20 antibody

Information sourced from Uniprot.org

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