• Immunofluorescence analysis of paraffin-embedded Human Colon using Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ11104411) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Human Colon CA using Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ11104411) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Cytokeratin 20 (KRT20) in paraffin-embedded Human colon using Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ11104411) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Cytokeratin 20 (KRT20) Rabbit polyclonal antibody (STJ11104411). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-KRT20 antibody (245-424) (STJ11104411)

SKU:
STJ11104411

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Keratin-Type I Cytoskeletal 20 (245-424) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT20
Gene ID: 54474
Uniprot ID: K1C20_HUMAN
Immunogen Region: 245-424
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 245-424 of human Cytokeratin 20 (KRT20) (NP_061883.1).
Immunogen Sequence: KYEVMAQKNLQEAKEQFERQ TAVLQQQVTVNTEELKGTEV QLTELRRTSQSLEIELQSHL SMKESLEHTLEETKARYSSQ LANLQSLLSSLEAQLMQIRS NMERQNNEYHILLDIKTRLE QEIATYRRLLEGEDVKTTEY QLSTLEERDIKKTRKIKTVV QEVVDGKVVSSEVKEVEENI
Tissue Specificity Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa.specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Post Translational Modifications Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury. Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine.
Protein Name Keratin - Type I Cytoskeletal 20
Cytokeratin-20
Ck-20
Keratin-20
K20
Protein It
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 20 antibody
Anti-Cytokeratin-20 antibody
Anti-Ck-20 antibody
Anti-Keratin-20 antibody
Anti-K20 antibody
Anti-Protein It antibody
Anti-KRT20 antibody

Information sourced from Uniprot.org

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