• IHC staining of human gullet cancer tissue, diluted at 1:200.
  • Immunofluorescence analysis of Rat-liver tissue. 1, CK16 monoclonal antibody (6F6) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Mouse-lung tissue. 1, CK16 monoclonal antibody (6F6) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-liver-cancer tissue. 1, CK16 monoclonal antibody (6F6) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, CK16 monoclonal antibody (6F6) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, CK16 monoclonal antibody (6F6) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-KRT16 antibody [6F6] (STJ96958)

SKU:
STJ96958

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin, type I cytoskeletal 16 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: 6F6
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 500-2000
1:200
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT16
Gene ID: 3868
Uniprot ID: K1C16_HUMAN
Specificity: The antibody detects endogenous CK16 proteins.
Immunogen: Synthetic Peptide of CK16
Function Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Protein Name Keratin - Type I Cytoskeletal 16
Cytokeratin-16
Ck-16
Keratin-16
K16
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 16 antibody
Anti-Cytokeratin-16 antibody
Anti-Ck-16 antibody
Anti-Keratin-16 antibody
Anti-K16 antibody
Anti-KRT16 antibody
Anti-KRT16A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance