• Immunofluorescence analysis of mouse skin using Cytokeratin 16 (KRT16) Rabbit polyclonal antibody (STJ29629) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat skin using Cytokeratin 16 (KRT16) Rabbit polyclonal antibody (STJ29629) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A-549 cells using Cytokeratin 16 (KRT16) Rabbit polyclonal antibody (STJ29629) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human skin using Cytokeratin 16 (KRT16) Rabbit polyclonal antibody (STJ29629) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Cytokeratin 16 (KRT16) Rabbit polyclonal antibody (STJ29629) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using Cytokeratin 16 (KRT16) antibody (STJ29629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-KRT16 antibody (190-430) (STJ29629)

SKU:
STJ29629

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KRT16 (190-430) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KRT16
Gene ID: 3868
Uniprot ID: K1C16_HUMAN
Immunogen Region: 190-430
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-430 of human Cytokeratin 16 (KRT16) (NP_005548.2).
Immunogen Sequence: LQIDNARLAADDFRTKYEHE LALRQTVEADVNGLRRVLDE LTLARTDLEMQIEGLKEELA YLRKNHEEEMLALRGQTGGD VNVEMDAAPGVDLSRILNEM RDQYEQMAEKNRRDAETWFL SKTEELNKEVASNSELVQSS RSEVTELRRVLQGLEIELQS QLSMKASLENSLEETKGRYC MQLSQIQGLIGSVEEQLAQL RCEMEQQSQEYQILLDVKTR LEQEIATYRRLLEGEDAHL
Tissue Specificity Expressed in the corneal epithelium (at protein level).
Function Epidermis-specific type I keratin that plays a key role in skin. Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance.
Protein Name Keratin - Type I Cytoskeletal 16
Cytokeratin-16
Ck-16
Keratin-16
K16
Database Links Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 16 antibody
Anti-Cytokeratin-16 antibody
Anti-Ck-16 antibody
Anti-Keratin-16 antibody
Anti-K16 antibody
Anti-KRT16 antibody
Anti-KRT16A antibody

Information sourced from Uniprot.org

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