Anti-KPNB1 antibody (300-400) (STJ111341)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KPNB1 (300-400) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000IHC-P:1:50-1:100IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KPNB1 |
| Gene ID | 3837 |
| Uniprot ID | IMB1_HUMAN |
| Immunogen Region | 300-400 |
| Immunogen Sequence | AAEQGRPPEHTSKFYAKGAL QYLVPILTQTLTKQDENDDD DDWNPCKAAGVCLMLLATCC EDDIVPHVLPFIKEHIKNPD WRYRDAAVMAFGCILEGPEP S |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KPNB1 (NP_002256.2). |
Additional Info
| Post Translational Modifications | Mono-ADP-ribosylated by PARP16. |
| Function | Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. Imports MRTFA, SNAI1 and PRKCI into the nucleus. (Microbial infection) In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. |
| Protein Name | Importin Subunit Beta-1Importin-90Karyopherin Subunit Beta-1Nuclear Factor P97Pore Targeting Complex 97 Kda SubunitPtac97 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-140342Reactome: R-HSA-1655829Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-180746Reactome: R-HSA-2995383Reactome: R-HSA-6798695Reactome: R-HSA-68616Reactome: R-HSA-909733Reactome: R-HSA-9615933Reactome: R-HSA-9636249Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmNucleus Envelope |
| Alternative Antibody Names | Anti-Importin Subunit Beta-1 antibodyAnti-Importin-90 antibodyAnti-Karyopherin Subunit Beta-1 antibodyAnti-Nuclear Factor P97 antibodyAnti-Pore Targeting Complex 97 Kda Subunit antibodyAnti-Ptac97 antibodyAnti-KPNB1 antibodyAnti-NTF97 antibody |
Information sourced from Uniprot.org