| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-KPNB1 (300-400) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000IHC-P 1:50-1:100IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
KPNB1 |
| Gene ID: |
3837 |
| Uniprot ID: |
IMB1_HUMAN |
| Immunogen Region: |
300-400 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KPNB1 (NP_002256.2). |
| Immunogen Sequence: |
AAEQGRPPEHTSKFYAKGAL QYLVPILTQTLTKQDENDDD DDWNPCKAAGVCLMLLATCC EDDIVPHVLPFIKEHIKNPD WRYRDAAVMAFGCILEGPEP S |
| Post Translational Modifications | Mono-ADP-ribosylated by PARP16. |
| Function | Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. In association with IPO7, mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus. |
| Protein Name | Importin Subunit Beta-1Importin-90Karyopherin Subunit Beta-1Nuclear Factor P97Pore Targeting Complex 97 Kda SubunitPtac97 |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-140342Reactome: R-HSA-1655829Reactome: R-HSA-168271Reactome: R-HSA-168276Reactome: R-HSA-180746Reactome: R-HSA-2995383Reactome: R-HSA-6798695Reactome: R-HSA-68616Reactome: R-HSA-909733Reactome: R-HSA-9615933Reactome: R-HSA-9636249Reactome: R-HSA-9692916 |
| Cellular Localisation | CytoplasmNucleus Envelope |
| Alternative Antibody Names | Anti-Importin Subunit Beta-1 antibodyAnti-Importin-90 antibodyAnti-Karyopherin Subunit Beta-1 antibodyAnti-Nuclear Factor P97 antibodyAnti-Pore Targeting Complex 97 Kda Subunit antibodyAnti-Ptac97 antibodyAnti-KPNB1 antibodyAnti-NTF97 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
