Anti-KPNA2 antibody (1-290) (STJ24341)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KPNA2 (1-290) antibody for use in WB, IP and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KPNA2 |
| Gene ID | 3838 |
| Uniprot ID | IMA1_HUMAN |
| Immunogen Region | 1-290 |
| Immunogen Sequence | MSTNENANTPAARLHRFKNK GKDSTEMRRRRIEVNVELRK AKKDDQMLKRRNVSSFPDDA TSPLQENRNNQGTVNWSVDD IVKGINSSNVENQLQATQAA RKLLSREKQPPIDNIIRAGL IPKFVSFLGRTDCSPIQFES AWALTNIASGTSEQTKAVVD GGAIPAFISLLASPHAHISE QAVWALGNIAGDGSVFRDLV IKYGAVDPLLALLAVPDMSS LACGYLRNLTWTLSNLCRN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human KPNA2 (NP_002257.1). |
Additional Info
| Tissue Specificity | Expressed ubiquitously. |
| Post Translational Modifications | Ubiquitinated. Deubiquitinated by USP22.leading to stabilization and increased activity. |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediator of PR-DUB complex component BAP1 nuclear import.acts redundantly with KPNA1 and Transportin-1/TNPO1. |
| Protein Name | Importin Subunit Alpha-1Karyopherin Subunit Alpha-2Rag Cohort Protein 1Srp1-Alpha |
| Database Links | Reactome: R-HSA-111932Reactome: R-HSA-1169408Reactome: R-HSA-168276Reactome: R-HSA-442729Reactome: R-HSA-5693548Reactome: R-HSA-9018519Reactome: R-HSA-9692916Reactome: R-HSA-9705671 |
| Cellular Localisation | CytoplasmNucleusEndoplasmic Reticulum MembraneGolgi Apparatus Membrane(Microbial Infection) Retained In Er/Golgi Membranes Upon Interaction With Sars-Cov Virus Orf6 Protein |
| Alternative Antibody Names | Anti-Importin Subunit Alpha-1 antibodyAnti-Karyopherin Subunit Alpha-2 antibodyAnti-Rag Cohort Protein 1 antibodyAnti-Srp1-Alpha antibodyAnti-KPNA2 antibodyAnti-RCH1 antibodyAnti-SRP1 antibody |
Information sourced from Uniprot.org