Anti-KLHDC2 antibody (306-406) (STJ117340)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal KLHDC2 (306-406) antibody for use in WB, IHC-P and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | KLHDC2 |
| Gene ID | 23588 |
| Uniprot ID | KLDC2_HUMAN |
| Immunogen Region | 306-406 |
| Immunogen Sequence | EWIQFNHPYTEKPRLWHTAC ASDEGEVIVFGGCANNLLVH HRAAHSNEILIFSVQPKSLV RLSLEAVICFKEMLANSWNC LPKHLLHSVNQRFGSNNTSG S |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 306-406 of human KLHDC2 (NP_055130.1). |
Additional Info
| Tissue Specificity | Widely expressed, with high levels in skeletal muscle, heart, pancreas and liver. Undetectable in peripheral blood leukocytes. |
| Post Translational Modifications | Autoubiquitinated by the CRL2(KLHDC2) E3 ligase complex. |
| Function | Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC2) complex specifically recognizes proteins with a diglycine (Gly-Gly) at the C-terminus, leading to their ubiquitination and degradation. The CRL2(KLHDC2) complex mediates ubiquitination and degradation of truncated SELENOK and SELENOS selenoproteins produced by failed UGA/Sec decoding, which end with a diglycine. The CRL2(KLHDC2) complex also recognizes proteolytically cleaved proteins ending with Gly-Gly, such as the N-terminal fragment of USP1, leading to their degradation. May also act as an indirect repressor of CREB3-mediated transcription by interfering with CREB3-DNA-binding. |
| Protein Name | Kelch Domain-Containing Protein 2Hepatocellular Carcinoma-Associated Antigen 33Host Cell Factor Homolog LcpHost Cell Factor-Like Protein 1Hclp-1 |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Kelch Domain-Containing Protein 2 antibodyAnti-Hepatocellular Carcinoma-Associated Antigen 33 antibodyAnti-Host Cell Factor Homolog Lcp antibodyAnti-Host Cell Factor-Like Protein 1 antibodyAnti-Hclp-1 antibodyAnti-KLHDC2 antibodyAnti-HCA33 antibody |
Information sourced from Uniprot.org