• Western blot analysis of extracts of PC-3 cells, using KLF6 antibody (STJ112051) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using KLF6 rabbit polyclonal antibody (STJ112051) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH/3T3 cells using KLF6 rabbit polyclonal antibody (STJ112051) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-KLF6 antibody (40-200) (STJ112051)

SKU:
STJ112051

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KLF6 (40-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KLF6
Gene ID: 1316
Uniprot ID: KLF6_HUMAN
Immunogen Region: 40-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 40-200 of human KLF6 (NP_001291.3).
Immunogen Sequence: ERYLQSEPCYVSASEIKFDS QEDLWTKIILAREKKEESEL KISSSPPEDTLISPSFCYNL ETNSLNSDVSSESSDSSEEL SPTAKFTSDPIGEVLVSSGK LSSSVTSTPPSSPELSREPS QLWGCVPGELPSPGKVRSGT SGKPGDKGNGDASPDGRRRV H
Tissue Specificity Highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. Weakly expressed in pancreas, lung, liver, heart and skeletal muscle. Also expressed in fetal brain, spleen and thymus.
Function Transcriptional activator. Binds a GC box motif. Could play a role in B-cell growth and development.
Protein Name Krueppel-Like Factor 6
B-Cell-Derived Protein 1
Core Promoter Element-Binding Protein
Gc-Rich Sites-Binding Factor Gbf
Proto-Oncogene Bcd1
Suppressor Of Tumorigenicity 12 Protein
Transcription Factor Zf9
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Krueppel-Like Factor 6 antibody
Anti-B-Cell-Derived Protein 1 antibody
Anti-Core Promoter Element-Binding Protein antibody
Anti-Gc-Rich Sites-Binding Factor Gbf antibody
Anti-Proto-Oncogene Bcd1 antibody
Anti-Suppressor Of Tumorigenicity 12 Protein antibody
Anti-Transcription Factor Zf9 antibody
Anti-KLF6 antibody
Anti-BCD1 antibody
Anti-COPEB antibody
Anti-CPBP antibody
Anti-ST12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance