• Western blot analysis of various lysates, using KIF2B Rabbit polyclonal antibody (STJ28563) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-KIF2B antibody (544-673) (STJ28563)

SKU:
STJ28563

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-KIF2B (544-673) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: KIF2B
Gene ID: 84643
Uniprot ID: KIF2B_HUMAN
Immunogen Region: 544-673
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 544-673 of human KIF2B (NP_115948.4).
Immunogen Sequence: NVDVRPYHRGHYPIGHEAPR MLKSHIGNSEMSLQRDEFIK IPYVQSEEQKEIEEVETLPT LLGKDTTISGKGSSQWLENI QERAGGVHHDIDFCIARSLS ILEQKIDALTEIQKKLKLLL ADLHVKSKVE
Tissue Specificity Highest level in lung. High level in ovary, moderate levels in heart, kidney, placenta, skeletal muscle and spleen (at protein level). Pancreas and spleen express a shorter isoform (at protein level).
Post Translational Modifications Phosphorylation at Thr-125 by PLK1 is required for activity in the correction of kinetochore-microtubules attachment errors, while phosphorylation at Ser-204 also by PLK1 is required for the kinetochore localization and activity in prometaphase.
Function Plus end-directed microtubule-dependent motor required for spindle assembly and chromosome movement. Has microtubule depolymerization activity. Plays a role in chromosome congression.
Protein Name Kinesin-Like Protein Kif2b
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2132295
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-5663220
Reactome: R-HSA-6811434
Reactome: R-HSA-68877
Reactome: R-HSA-9648025
Reactome: R-HSA-983189
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Chromosome
Centromere
Kinetochore
Association With Kinetochore Is Transient
Alternative Antibody Names Anti-Kinesin-Like Protein Kif2b antibody
Anti-KIF2B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance