• Anti-Keratin 14 antibody [EBS-IF-004] (STJ16101021)
  • Anti-Keratin 14 antibody [EBS-IF-004] (STJ16101021)

Anti-Keratin 14 antibody [EBS-IF-004] (STJ16101021)

SKU:
STJ16101021

£264.50 - £1,448.50
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Host: Mouse
Applications: FC/WB/IF/IHC
Reactivity: Cat/Human/Pig/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Keratin 14 is suitable for use in Flow Cytometry, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: EBS-IF-004
Conjugation: Unconjugated
Isotype: IgMk
Formulation: PBS with 0.02% Sodium Azide.
Purification: Affinity purified from tissue culture.
Concentration: Can be provided as 100 µg/mL, 500 µg/mL or 1mg/mL.
Dilution Range: Flow Cytometry (1-2 µg/million cells in 0.1 ml, fix cells in 4% PFA for 10 min, at 4°C, permeabilize with 0, 2% saponin or digitonin for 15 min, at 4°C). Immunoblotting (1-2 µg/ml). Immunofluorescence (1-2 µg/ml). Immunohistology (formalin-fixed: 2-4
Storage Instruction: Store for up to 1 year at 2-8°C upon receipt.
Gene Symbol: KRT14
Gene ID: 3861
Uniprot ID: K1C14_HUMAN
Immunogen: A hybrid (BALB/c x B6) mouse was immunized with AA 459-472 at the carboxyterminal end of human cytokeratin 14, conjugated to KLH. Fusion partner: SP2/0.
Tissue Specificity Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
Post Translational Modifications A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Protein Name Keratin - Type I Cytoskeletal 14
Cytokeratin-14
Ck-14
Keratin-14
K14
Database Links Reactome: R-HSA-446107
Reactome: R-HSA-6805567
Reactome: R-HSA-6809371
Cellular Localisation Cytoplasm
Nucleus
Expressed In Both As A Filamentous Pattern
Alternative Antibody Names Anti-Keratin - Type I Cytoskeletal 14 antibody
Anti-Cytokeratin-14 antibody
Anti-Ck-14 antibody
Anti-Keratin-14 antibody
Anti-K14 antibody
Anti-KRT14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance