Anti-Keap1 antibody (500-550) {FITC} (STJ506356)

SKU:
STJ506356-100

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Host: Rabbit
Applications: ELISA/IP/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Keap1 (500-550) is suitable for use in ELISA, Immunoprecipitation and Western Blot research applications.
Clonality: Polyclonal
Conjugation: FITC
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.5-1.5 µg/µl
Dilution Range: ELISA 1:10, 000
IP 1:200
WB 1:500
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: KEAP1
Gene ID: 9817
Uniprot ID: KEAP1_HUMAN
Immunogen Region: 500-550
Immunogen: Synthetic peptide corresponding to amino acids within region 500-550 on human Kelch-like ECH-associated protein 1 protein.
Tissue Specificity Broadly expressed, with highest levels in skeletal muscle.
Post Translational Modifications Non-enzymatic covalent modifications of reactive cysteines by electrophile metabolites inactivate the BCR(KEAP1) complex. Accumulation of fumarate promotes the formation of cysteine S-succination (S-(2-succinyl)cysteine), leading to inactivate the BCR(KEAP1) complex and promote NFE2L2/NRF2 nuclear accumulation and activation. Nitric oxide-dependent 8-Nitro-cGMP formation promotes cysteine guanylation (S-cGMP-cysteine), leading to NFE2L2/NRF2 nuclear accumulation and activation. Itaconate, an anti-inflammatory metabolite generated in response to lipopolysaccharide, alkylates cysteines, activating NFE2L2/NRF2. Methylglyoxal, a reactive metabolite that accumulates when the glycolytic enzyme PGK1 is inhibited, promotes formation of a methylimidazole cross-link between proximal Cys-151 and Arg-135 on another KEAP1 molecule, resulting in an inactive dimer that inactivates the BCR(KEAP1) complex. Degraded via a proteasomal-independent process during selective autophagy: interaction with phosphorylated SQSTM1/p62 sequesters KEAP1 in inclusion bodies, leading to its degradation. Auto-ubiquitinated by the BCR(KEAP1) complex. Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination. Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress.
Function Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting NFE2L2/NRF2 for ubiquitination. KEAP1 acts as a key sensor of oxidative and electrophilic stress: in normal conditions, the BCR(KEAP1) complex mediates ubiquitination and degradation of NFE2L2/NRF2, a transcription factor regulating expression of many cytoprotective genes. In response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex, promoting NFE2L2/NRF2 nuclear accumulation and expression of phase II detoxifying enzymes. In response to selective autophagy, KEAP1 is sequestered in inclusion bodies following its interaction with SQSTM1/p62, leading to inactivation of the BCR(KEAP1) complex and activation of NFE2L2/NRF2. The BCR(KEAP1) complex also mediates ubiquitination of SQSTM1/p62, increasing SQSTM1/p62 sequestering activity and degradation. The BCR(KEAP1) complex also targets BPTF and PGAM5 for ubiquitination and degradation by the proteasome.
Protein Name Kelch-Like Ech-Associated Protein 1
Cytosolic Inhibitor Of Nrf2
Inrf2
Kelch-Like Protein 19
Database Links Reactome: R-HSA-5689880
Reactome: R-HSA-8951664
Reactome: R-HSA-9679191
Reactome: R-HSA-9755511
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Nucleus
Mainly Cytoplasmic
In Response To Selective Autophagy
Relocalizes To Inclusion Bodies Following Interaction With Sqstm1/P62
Alternative Antibody Names Anti-Kelch-Like Ech-Associated Protein 1 antibody
Anti-Cytosolic Inhibitor Of Nrf2 antibody
Anti-Inrf2 antibody
Anti-Kelch-Like Protein 19 antibody
Anti-KEAP1 antibody
Anti-INRF2 antibody
Anti-KIAA0132 antibody
Anti-KLHL19 antibody

Information sourced from Uniprot.org

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