• STJ73601 (0. 1µg/ml) staining of Human Cerebellum (A) + Peptide (B) , NIH3T3 (C) and negative Control Adrenal Gland (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ73601 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73601 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight at 4°C (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-KCNN4/KCa3.1 antibody (Internal) (STJ73601)

SKU:
STJ73601-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF/FC/IHC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-KCNN4/KCa3.1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IHC-3.75µg/ml
IF-Strong expression of the protein seen in the cytoplasm/membranes of HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: KCNN4
Gene ID: 3783
Uniprot ID: KCNN4_HUMAN
Immunogen Region: Internal
Accession Number: NP_002241.1
Immunogen Sequence: ERQAVNATGHLSD
Post Translational Modifications Phosphorylation at His-358 by NDKB activates the channel, and conversely it's dephosphorylation by PHPT1 inhibits the channel.
Function Forms a voltage-independent potassium channel that is activated by intracellular calcium. Activation is followed by membrane hyperpolarization which promotes calcium influx. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis.
Protein Name Intermediate Conductance Calcium-Activated Potassium Channel Protein 4
Sk4
Skca 4
Skca4
Ikca1
Ik1
Kca3.1
Kca4
Putative Gardos Channel
Database Links Reactome: R-HSA-1296052
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Intermediate Conductance Calcium-Activated Potassium Channel Protein 4 antibody
Anti-Sk4 antibody
Anti-Skca 4 antibody
Anti-Skca4 antibody
Anti-Ikca1 antibody
Anti-Ik1 antibody
Anti-Kca3.1 antibody
Anti-Kca4 antibody
Anti-Putative Gardos Channel antibody
Anti-KCNN4 antibody
Anti-IK1 antibody
Anti-IKCA1 antibody
Anti-KCA4 antibody
Anti-SK4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance