| Host: |
Goat |
| Applications: |
Pep-ELISA/WB/IF/FC/IHC |
| Reactivity: |
Human/Mouse/Rat/Dog |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-KCNN4/KCa3.1 (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in Human Cerebellum lysates and in lysates of cell line NIH3T3 (calculated MW of 47.7kDa, according to Human NP_002241.1 and Mouse NP_032459.3). An additiona |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
KCNN4 |
| Gene ID: |
3783 |
| Uniprot ID: |
KCNN4_HUMAN |
| Immunogen Region: |
Internal |
| Accession Number: |
NP_002241.1 |
| Immunogen Sequence: |
ERQAVNATGHLSD |
| Post Translational Modifications | Phosphorylation at His-358 by NDKB activates the intermediate conductance calcium-activated potassium channel activity, and conversely it's dephosphorylation by PHPT1 inhibits this activity. |
| Function | Intermediate conductance calcium-activated potassium channel that mediates the voltage-independent transmembrane transfer of potassium across the cell membrane through a constitutive interaction with calmodulin which binds the intracellular calcium allowing its opening. The current is characterized by a voltage-independent activation, an intracellular calcium concentration increase-dependent activation and a single-channel conductance of about 25 picosiemens. Also presents an inwardly rectifying current, thus reducing its already small outward conductance of potassium ions, which is particularly the case when the membrane potential displays positive values, above + 20 mV. Controls calcium influx during vascular contractility by being responsible of membrane hyperpolarization induced by vasoactive factors in proliferative vascular smooth muscle cell types. Following calcium influx, the consecutive activation of KCNN4 channel leads to a hyperpolarization of the cell membrane potential and hence an increase of the electrical driving force for further calcium influx promoting sustained calcium entry in response to stimulation with chemotactic peptides. Required for maximal calcium influx and proliferation during the reactivation of naive T-cells. Plays a role in the late stages of EGF-induced macropinocytosis through activation by PI(3)P. |
| Protein Name | Intermediate Conductance Calcium-Activated Potassium Channel Protein 4Skca 4Skca4Hsk4Gardos ChannelIkca1Hik1Kca3.1Putative Gardos ChannelHkca4 |
| Database Links | Reactome: R-HSA-1296052 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionRuffle MembraneTargeted To Membrane Ruffles After Egf Stimulation |
| Alternative Antibody Names | Anti-Intermediate Conductance Calcium-Activated Potassium Channel Protein 4 antibodyAnti-Skca 4 antibodyAnti-Skca4 antibodyAnti-Hsk4 antibodyAnti-Gardos Channel antibodyAnti-Ikca1 antibodyAnti-Hik1 antibodyAnti-Kca3.1 antibodyAnti-Putative Gardos Channel antibodyAnti-Hkca4 antibodyAnti-KCNN4 antibodyAnti-IK1 antibodyAnti-IKCA1 antibodyAnti-KCA4 antibodyAnti-SK4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
